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CROP-Seq in Primary Human T Cells

ABSTRACT: We adapted sgRNA lentiviral infection and Cas9 electroporation (SLICE) to allow for CROP-Seq (Datlinger et al, Nat Med 2017) in primary cells. We used a library of 48 sgRNA, derived from GW screens, to explore transcriptional changes downstream of CRISPR-KO.

ORGANISM(S): Homo sapiens

PROVIDER: GSE119450 | GEO | 2018/09/06

REPOSITORIES: GEO

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