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M6A-seq profiling in WT, WtapKO and Mettl3KO mESCs

ABSTRACT: By m6A-seq, we examined the m6A pattern on Xist, which immediately after the A-repeat region. The cell line, integrating multiple copy of Xist on chr3, with m6A core machinery knockout (Wtap and Mettl3) were generated. We also profiled m6A-seq in transgenic Xist line, and observed a global reduction of m6A level, validated by m6A-LC-MS/MS. Unexpectedly, we haven’t observed reduced m6A level on Xist m6A region downstream of A-repeat in transgenic Xist line.

ORGANISM(S): Mus musculus

PROVIDER: GSE119487 | GEO | 2019/06/17

REPOSITORIES: GEO

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