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HOIL-1/RBCK1 promotes p53 degradation in Renal cancer cells

ABSTRACT: We performed RBCK1 depletion in renal cancer cells along with RNA sequencing. The expression profiling showed p53 signaling as a potential RBCK1 target and the conclusions were also verified in clinical samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE119864 | GEO | 2019/03/28

REPOSITORIES: GEO

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HOIL-1/RBCK1 promotes p53 degradation in Renal cancer cells

Project description:HOIL-1/RBCK1 promotes p53 degradation in Renal cancer cells

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RBCK1 promotes p53 degradation via ubiquitination in renal cell carcinoma.

Project description:Renal cell carcinoma (RCC) accounts for approximately 3% of adult malignancies, and the incidence of RCC continues to rise worldwide. Although RCC can be treated with surgery at an early stages, the five-year survival rates have been observed to decline dramatically in patients with advanced disease. Most patients with RCC treated with cytotoxic or targeted drugs will develop resistance at some point during therapy. Thus, it is necessary to identify novel therapeutic targets for RCC. Here, we found that RANBP2-type and C3HC4-type zinc finger-containing 1 (RBCK1) expression was upregulated in human RCC samples. Analysis of multiple public databases revealed the correlation between RBCK1 expression and poor prognosis in RCC patients. Subsequently, we performed RBCK1 depletion experiments in RCC cells that severely affected the in vivo and in vitro proliferation of renal cancer cells. The effects of RBCK1 on cell proliferation could be rescued with p53 expression knockdown in two cell lines expressing wild-type p53. Further experiments demonstrated that RBCK1 could facilitate p53 poly-ubiquitination and degradation by direct interaction with p53. Together, our results show that RBCK1 may serve as a promising target for RCC therapy by restoring p53 functions.

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PML restrains p53 activity and cellular senescence in clear cell renal cell carcinoma

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P53-P21-RB pathway promotes BRD4 degradation in liver cancer through decreasing USP1 transcription

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The RNF26/CBX7 axis modulates the TNF signaling pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC

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Targeted single-cell RNA-seq identifies minority cell types of kidney distal nephron & A Comprehensive Map of mRNAs and Their Isoforms across All 14 Renal Tubule Segments of Mouse

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