Transcriptomics

Dataset Information

0

Multiple genes recruited from hormone pathways partition maize diterpenoid defences.


ABSTRACT: Duplication and divergence of primary pathway genes underlie the evolution of plant specialized metabolism; however, mechanisms partitioning parallel hormone and defence pathways are often speculative. For example, the primary pathway intermediate ent-kaurene is essential for gibberellin biosynthesis and is also a proposed precursor for maize antibiotics. By integrating transcriptional coregulation patterns, genome-wide association studies, combinatorial enzyme assays, proteomics and targeted mutant analyses, we show that maize kauralexin biosynthesis proceeds via the positional isomer ent-isokaurene formed by a diterpene synthase pair recruited from gibberellin metabolism. The oxygenation and subsequent desaturation of ent-isokaurene by three promiscuous cytochrome P450s and a new steroid 5α reductase indirectly yields predominant ent-kaurene-associated antibiotics required for Fusarium stalk rot resistance. The divergence and differential expression of pathway branches derived from multiple duplicated hormone-metabolic genes minimizes dysregulation of primary metabolism via the circuitous biosynthesis of ent-kaurene-related antibiotics without the production of growth hormone precursors during defence.

ORGANISM(S): Zea mays

PROVIDER: GSE120135 | GEO | 2019/07/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-09-08 | GSE96954 | GEO
2012-02-12 | E-GEOD-35729 | biostudies-arrayexpress
2012-02-12 | GSE35729 | GEO
2019-12-31 | GSE94012 | GEO
2009-03-07 | E-GEOD-15048 | biostudies-arrayexpress
2009-03-07 | GSE15048 | GEO
2024-09-02 | BIOMD0000000422 | BioModels
2021-11-15 | GSE188626 | GEO
2024-11-10 | GSE280929 | GEO
2016-02-01 | E-MTAB-3869 | biostudies-arrayexpress