Screening of the targeted mRNAs of ER-anchored PARN by transcriptome sequencing.
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ABSTRACT: The goals of this study are to identify the potential targets and then elucidate the function of ER-anchored PARN. After overexpressing the empty vector, PARN(wild type) or D28A(the enzyme-dead mutant of PARN) in HEK-293T cell respectively and the following fractionation of the cytosol and ER fraction, we performed the high-throughput RNA sequencing for each fraction. After comparing the abundance of detected transcripts in different samples we found ER-anchored PARN only affects a small subset of mRNAs in HEK-293T cell.
ORGANISM(S): Homo sapiens
PROVIDER: GSE120209 | GEO | 2021/09/19
REPOSITORIES: GEO
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