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Identification of potential miR-215 target genes in patient-derived tumor-initiating cell (TIC) cultures

ABSTRACT: Stable overexpression of miR-215 in patient-derived TIC culture T51 The main focus of the study was the identification and validation of potential target genes of hypoxia-responsive miR-215 in colon TICs

ORGANISM(S): Homo sapiens

PROVIDER: GSE120422 | GEO | 2019/09/17

REPOSITORIES: GEO

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Identification of differentially expressed miRNAs in hypoxic tumor-initiating cell (TIC) cultures

Project description:Patient-derived TIC cultures T6 and T18 were used to study the effect of normoxic vs. hypoxic culture conditions The main focus of the study was the identification and validation of new hypoxia-responsive miRNAs and target genes in such colon TICs

2019-09-17 | GSE120424 | GEO

Identification of potential miR-215 target genes in patient-derived tumor-initiating cell (TIC) cultures

Project description:Identification of potential miR-215 target genes in patient-derived tumor-initiating cell (TIC) cultures

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Expression data from highly purified MMTV-Neu Tumor Initiating Cells (TICs) and the non-TIC CD24- fraction

Project description:The cancer stem cell model maintains that tumors are organized in a hierarchy driven by tumor initiating cells (TICs), and that patient survival inversely correlates with TIC gene expression. Here we generated a prognostic signature for HER2+ breast cancer from TICs purified from MMTV-Her2/Neu mammary tumors. TICs from this model, identified as Lin-:CD24+:JAG1- at a frequency of 2-5% by serial and single cell transplantation assays, showed elevated expression of proliferation genes and low expression of differentiation genes (compared to non-TIC fraction CD24- of the same tumor). We used microarrays to detect differentially expressed genes in the TIC fraction compared to the non-TIC fraction of the same tumor

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Expression data from highly purified MMTV-Neu Tumor Initiating Cells (TICs) and the non-TIC CD24- fraction

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Gene expression of primary MMTV-Neu tumors compared to secondary tumors generated from lin- and single tumor initiating cell (TIC) transplantation

Project description:Most solid tumors seem to be organized in a hierarchy in which only a fraction of cells, termed tumor-initiating cells (TICs), is capable of disseminating new tumors after transplantation into recipient mice. However, whether a single TIC can induce a tumor or whether it requires additional TICs or non-TICs for tumor initiation is not known. Here we show that injections of single CD24+:Jag1- cells from Her2/Neu+ mammary tumors into recipient mammary glands induced tumors at a frequency of 1/22. Single cell-derived secondary tumors exhibited histology, flow cytometry and global expression profiles that were indistinguishable from primary tumors. Thus, a single TIC can act cell autonomously to induce a tumor and therefore complete eradication of all TICs would be required to cure cancer. Total RNA obtained from primary MMTV-Neu tumors, secondary tumors generated by lin- cell transplantation, and secondary tumors generated by a single TIC, were used for microarray analysis to determine differentially regulated genes in the secondary tumors. Cluster analysis based on gene expression were then performed to assess tumor similarity

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Gene expression of primary MMTV-Neu tumors compared to secondary tumors generated from lin- and single tumor initiating cell (TIC) transplantation

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Micro-RNAs from Plasma-Derived Small Extracellular Vesicles as Potential Biomarkers for Tic Disorders Diagnosis

Project description:tic disorders (TDs) are a series of childhood neuropsychiatric disorders characterized by invol-untary motor and/or vocal tics and commonly comorbid with several other psychopathological and/or behavioral disorders. In the present study, we aimed to identify serum small extracellular vesicles (sEVs) derived microRNAs (miRNAs) from the serum samples of children for Tic Disorders Diagnosis

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Tumor-initiating cell-specific miR-1246 and miR-1290 converge to promote non-small cell lung cancer progression [Illumina]

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2022-06-07 | PXD032958 | Pride

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