Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Selective eradication of leukemia cells with reprogramming factors (RNA-Seq)

ABSTRACT: We used RNA-seq to evaluate the global programme of gene expression after Dox inducible Yamanaka factors ectopic expression during this biological process.

ORGANISM(S): Mus musculus

PROVIDER: GSE121122 | GEO | 2019/09/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Selective eradication of leukemia cells with reprogramming factors (ATAC-Seq)

Project description:We used ATAC-seq to evaluate the global programme of chromtain accessibility after Dox inducible Yamanaka factors ectopic expression during this biological process.

2019-09-23 | GSE121121 | GEO

Eradiation of leukemia by effective reprogramming-associated cell elimination (ERACE)

Project description:We used microarrays to evaluate the global programming of gene expression after Dox inducible Yamanaka factors ectopic expression and identified distinct classes of genes during this biological process in vivo.

2018-02-01 | GSE78739 | GEO

Selective elimination of leukemia cells by reprogramming factors

Project description:To investigate the molecular mechanism for the elimination of leukemia cells after Dox induced OSKM expression, we sorted MLL-AF9 leukemia cells from Dox untreated and treated groups (48h and 72h) leukemic mice for gene expression profiling analysis.

2019-09-23 | GSE64414 | GEO

Selective eradication of leukemia cells with reprogramming factors

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-09-21 | GSE121123 | GEO

Selective eradication of leukemia cells with reprogramming factors

Project description:Selective eradication of leukemia cells with reprogramming factors

| PRJNA495802 | ENA

Targeting of apoptosis gene loci by reprogramming factors leads to selective eradication of leukemia cells.

Project description:Applying somatic cell reprogramming strategies in cancer cell biology is a powerful approach to analyze mechanisms of malignancy and develop new therapeutics. Here, we test whether leukemia cells can be reprogrammed in vivo using the canonical reprogramming transcription factors-Oct4, Sox2, Klf4, and c-Myc (termed as OSKM). Unexpectedly, we discover that OSKM can eradicate leukemia cells and dramatically improve survival of leukemia-bearing mice. By contrast, OSKM minimally impact normal hematopoietic cells. Using ATAC-seq, we find OSKM induce chromatin accessibility near genes encoding apoptotic regulators in leukemia cells. Moreover, this selective effect also involves downregulation of H3K9me3 as an early event. Dissection of the functional effects of OSKM shows that Klf4 and Sox2 play dominant roles compared to c-Myc and Oct4 in elimination of leukemia cells. These results reveal an intriguing paradigm by which OSKM-initiated reprogramming induction can be leveraged and diverged to develop novel anti-cancer strategies.

| S-EPMC6898631 | biostudies-literature

Selective eradication of leukemia cells with reprogramming factors (RNA-Seq)

Project description:Selective eradication of leukemia cells with reprogramming factors (RNA-Seq)

| PRJNA495803 | ENA

Selective eradication of leukemia cells with reprogramming factors (ATAC-Seq)

Project description:Selective eradication of leukemia cells with reprogramming factors (ATAC-Seq)

| PRJNA495799 | ENA

Aberrant DNA methylation reprogramming during iPS cell generation is dependent on the choice of reprogramming factors

Project description:we generate iPSCs from a common fibroblast cell source using either the Yamanaka factors (OCT4, SOX2, KLF4 and MYC) or the Thomson factors (OCT4, SOX2, NANOG and LIN28) and determined their genome-wide DNA methylation profiles. In addition to shared DNA methylation aberrations present in all our iPSCs, we identify Yamanaka-iPSC (Y-iPSCs)-specific and Thomson-iPSCs (T-iPSC)-specific recurrent aberrations. Bisulphite converted DNA from 9 iPS cells derived using Yamanaka factors (OSKM), 6 iPS cells derived using Thompson factors (OSLN), 2 parental fibroblasts and one embrionic ES cell were hybridised to the Illumina Infinium 450k Human Methylation Beadchip

2014-01-16 | E-GEOD-54115 | biostudies-arrayexpress

Genome-wide transcriptomes of adult mouse cardiomyocytes (CMs) with ectopic expression of reprogramming factors

Project description:We carried out bulk RNA sequencing of normal adult mouse CMs (Control-CMs) and adult mouse CMs with ectopic overexpression of Yamanaka factors (Oct4, Sox2, Klf4, Myc, OSKM-CMs), based on a hypothesis that OSKM would induce at least partial CM dedifferentiation in vivo. More than 2,000 genes were differentially expressed between Control- and OSKM-CMs, which were enriched for gene ontology (GO) terms related to multiple key aspects of CM dedifferentiation. This study provides a genome-wide transcriptional profile of dedifferentiating CMs induced by OSKM.

2024-04-09 | GSE172192 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data