Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human osteoblasts transfected with miR-320a

ABSTRACT: Analysis of gene expression of primary osteoblasts transfected with miR-320a mimic or miR-320a inhibitor or their respective controls.

ORGANISM(S): Homo sapiens

PROVIDER: GSE121892 | GEO | 2018/11/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Human osteoblasts transfected with miR-320a

Project description:Human osteoblasts transfected with miR-320a

| PRJNA498995 | ENA

Expression profiling of primary calvaria osteoblasts treated with miR-195 mimic, rhBMP2 and at different stages of differentiation

Project description:Primary calvaria osteoblasts from newborn mice were cultured. Cells were transfected with control and miR-195 mimic. Following serum starvation cells were treated with recombinant human BMP2 and expression was analyzed 4 hours after treatment. Additionally, osteoblast differentiation was induced with osteogenic medium. Cells were analyzed at confluence (day 0) and 9 days later (day 9).

2014-12-02 | E-MTAB-2450 | biostudies-arrayexpress

mRNA profiling of MCF7 cells transfected with a miR-139-5p mimic

Project description:Gene expression profiling of MCF7 cells transfected with a miR-139-5p or negative control mimic

2017-12-08 | GSE107800 | GEO

Transcriptional profile comparing Hep3B transfected with miR-19b inhibitor with Hep3B transfected with control

Project description:miR-19b was up-regulated with hepatocellular carcinoma. We compared the transcriptional profile of Hep 3B transfected with miR-19b inhibitor with Hep3B transfected with control to identify genes affected by miR-19b knockdown.

2015-07-22 | GSE69519 | GEO

Real-time quantitative PCR analysis of miRNA in mouse peritoneal macrophages upon miR-33 or miR-33* modulation

Project description:Mouse peritoneal macrophages were transfected with 80-120 nM miRIDIAN miRNA mimics (miR-mimic-33/miR-mimic-33*) or with 80-120 nM miRIDIAN miRNA inhibitors (anti-miR-33 ASO/anti-miR-33*ASO) Control samples were treated with an equal concentration of a non-targeting control mimics sequence (control mimic) or inhibitor negative control sequence (control aso), to control for non-specific effects in miRNA experiments.

2016-04-18 | GSE79055 | GEO

Effect of miR-125b and its isomiRs on gene expression subjected to hypoxic challenge

Project description:To investigate the gene expresiion regulated by miR-125b and its isomiRs. We transfected H9C2 cells with respective mimics and performed gene expression profiling anlaysis using data from RNA-seq of mimic controls, miR-125b and its isomiRs mimics subjected to normoxia and hypoxia treatment.

2023-11-22 | GSE227958 | GEO

Expression Profiling of U2OS Cell Lines Transfected with MicroRNA-571 Mimic or MicroRNA-571 Inhibitor vs. MicroRNA-Control Transfected U2OS Cell Lines

Project description:Osteosarcoma U2OS cells were studied to profile the expression of miR-571 mimic & inhibitor transfected cells. The goal was to gain insights or to build hypotheses into the mechanisms of miR-571 regulation effects on the DNA replication network in cell-based model.

2020-02-23 | GSE118998 | GEO

Human Papillary thyroid cancer cell line, BCPAP control vs. miR-146b-5p mimic transfected

Project description:Transcriptional profiling of human papillary thyroid cancer cells comparing control untreated BCPAP cells with BCPAP cells transfected with miR-145b-5p mimic.

2015-12-17 | GSE76050 | GEO

RIP: miR-202 mimic-transfected HeLa cells vs. negative control

Project description:Potential miR-202 targets were identified using a targetome-wide RIP-based microarray. HeLa cells were transfected with either a miR-202 mimic or a scrambled single-stranded RNA negative control. RNA was isolated from total cell lysate prior to IP and from antibody-immobilized Protein G agarose beads-RNP complexes (post-IP).

2012-12-19 | GSE42981 | GEO

Sequencing of Argonaute-bound miRNA/mRNA hybrids reveals regulation of the unfolded protein response by microRNA-320a

Project description:MicroRNAs (miRNA) are short non-coding RNAs widely implicated in development, gene regulation, and disease progression. Most miRNAs utilize the RNase III enzymes Drosha and Dicer for biogenesis in animals. One notable exception is the RNA polymerase II transcription start sites (TSS) miRNAs whose biogenesis requires Dicer but not Drosha. The functional importance of the TSS-miRNA biogenesis pathway has remained uncertain due to their unelucidated targetomes. To better understand the function of TSS-miRNAs, we applied a modified Crosslinking, Ligation, and Sequencing of Hybrids on Argonaute (Ago-qCLASH) to identify the targets for TSS-miRNAs in HCT116 colorectal cancer cells with or without Drosha knockout (KO). We observed that miR-320a hybrids dominate in TSS-miRNA hybrids identified by Ago-qCLASH. Targets for miR-320a are enriched in the eIF2 signaling pathway, a downstream component of the unfolded protein response. Consistently, in miR-320a mimic- and inhibitor- transfected cells, differentially expressed genes are enriched in the eIF2 signaling pathway. Within the Ago-qCLASH data, we identified the endoplasmic reticulum (ER) chaperone Calnexin as a direct miR-320a target, thus connecting miR-320a to the unfolded protein response. During ER stress, but not amino acid deprivation, miR-320a up-regulates ATF4, a critical transcription factor for resolving ER stress. Our study helps to elucidate the targetome of the TSS-miRNAs in colorectal cancer cells and establishes miR-320a as a regulator of unfolded protein response.

2021-06-23 | GSE164634 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data