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Gene expression profiling among human embryonic stem cells, differerentiated EBs and adult cells

ABSTRACT: Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. However, much work needs to be done to understand the molecular mechanisms responsible for the maintenance of the undifferentiatiation state and the differentiation process of human embryonic stem cells. Keywords: cell type comparison design

ORGANISM(S): Homo sapiens

PROVIDER: GSE12228 | GEO | 2009/04/17

SECONDARY ACCESSION(S): PRJNA113859

REPOSITORIES: GEO

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Gene expression profiling among human embryonic stem cells, differerentiated EBs and adult cells

Project description:Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. However, much work needs to be done to understand the molecular mechanisms responsible for the maintenance of the undifferentiatiation state and the differentiation process of human embryonic stem cells. Keywords: cell type comparison design We performed gene expression profiling on 3 separate passages from 3 different hES cell lines (WA09 (H9), TE06 (I6) and BG01v), EBs samples derived from WA09 cells at 3 different time points, and 5 types of adult cells (HUVEC, HMVEC, UASMC NHA, and LFB).

2009-04-17 | E-GEOD-12228 | biostudies-arrayexpress

microRNA expression profiling among human embryonic stem cells, differerentiated EBs and adult cells

2009-04-17 | GSE12229 | GEO

microRNA expression profiling among human embryonic stem cells, differerentiated EBs and adult cells

Project description:Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. We aimed to identify the unique signature of miRNAs in human embryonic stem cells. Keywords: cell type comparison design We performed microRNA expression profiling on 3 passages from 2 different hES cell lines (WA09 (H9) and BG01v) and 4 passages from 1 hES cell line (TE06 (I6)), EBs samples derived from WA09 cells at 4 different time points with replicates, and 5 types of adult cells (HUVEC, HMVEC, UASMC NHA, and LFB).

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Transcription profiling of human embryonic stem cell differentiation

Project description:MicroRNAs (miRNAs) are noncoding RNAs of approximately 22 nucleotides in length that usually suppress the translation of target messenger RNAs (mRNAs) through partial complementarity to the 3Â¡Â¦ untranslated region (3Â¡Â¦ UTR) of protein-coding mRNAs in animals. However, there is increasing evidence that miRNAs can also reduce the steady-state levels of their target mRNAs in animals. In this investigation, both miRNA and mRNA profiles from the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF) were quantitatively determined. Three common target genes of three highly expressed hES cell-specific miRNAs were identified by inverse expression levels of miRNAs to their target mRNAs. Experiment Overall Design: Three types of human embryonic stem cells and their derived cells (the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF)) were selected for RNA extraction. The genome-wide mRNA expression of T3ES, T3EB and T3DF cells was determined using Affymetrix human genome U133 plus 2.0 GeneChip. The abundantly expressed genes (10-fold above overall mean) in T3ES, T3EB and T3DF cells are selected to compare with the predicted gene targets of differentially expressed miRNAs in the same set of cells.

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