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T cell receptor-triggered nuclear actin network formation drives CD4 T cell effector functions

ABSTRACT: T cell antigen receptor (TCR) signaling triggers selective cytokine expression to drive T cell proliferation and differentiation required for immune defense and surveillance. The nuclear signaling events responsible for specificity in cytokine gene expression upon T cell activation are largely unknown. Here, we uncover formation of a dynamic actin filament network in the nucleus that regulates cytokine expression for effector functions of CD4 T lymphocytes. TCR engagement triggers the rapid and transient formation of a nuclear actin filament network via nuclear Arp2/3 complex, induced by elevated nuclear Ca2+ levels and regulated via N-Wasp and NIK. Specific interference with TCR-induced formation of nuclear actin filaments impairs production of effector cytokines and prevents generation of antigen-specific antibodies, but does not interfere with immune synapse formation and cell proliferation. Ca2+-regulated actin polymerization in the nucleus allows CD4 T cells the rapid conversion of TCR signals into effector functions required for T cell help Identification of nuclear F-actin sensitive genes upon TCR signaling

ORGANISM(S): Homo sapiens

PROVIDER: GSE122531 | GEO | 2018/11/15

REPOSITORIES: GEO

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T cell receptor-triggered nuclear actin network formation drives CD4 T cell effector functions

Project description:T cell receptor-triggered nuclear actin network formation drives CD4 T cell effector functions

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