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Coordinate regulation of expression of SdsR toxin and its downstream pphA gene by RyeA antitoxin expression in Escherichia coli

ABSTRACT: RNA-seq analysis of cells with an ryeA or sdsR promoter mutation In Escherichia coli, SdsR and RyeA, a unique pair of mutually cis-encoded sRNAs, act as toxin and antitoxin, respectively. They are located in the same intergenic region, but transcribed in bidirectional way. Expression of SdsR is reciprocally related to that of RyeA; however, it remains unclear how their syntheses are regulated by each other. Here, using sdsR and ryeA promoter mutant strains, we characterized biosynthesis of the two sRNAs during growth and their coordinate regulation. RyeA transcription occurs even when cells enter the stationary phase, but its apparent expression is restricted to exponentially growing cells because of its degradation by SdsR. The appearance of SdsR was similarly delayed due to its RyeA-mediated degradation. We also found that the sdsR promoter was mostly responsible for transcription of the downstream pphA gene encoding a phosphatase and that pphA mRNA was synthesized through transcriptional read-through over the sdsR terminator. Transcription from the sigma70-dependent ryeA promoter inhibited transcription from the ?S-dependent sdsR promoter through transcriptional interference. This transcription inhibition also downregulated pphA expression, but RyeA itself did not downregulate pphA expression. Our results show that expression of the toxin SdsR and its downstream pphA gene are regulated by expression of the antitoxin RyeA, but differently.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE122921 | GEO | 2019/06/30

REPOSITORIES: GEO

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