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Transcriptome of non-involved lung parenchyma from ever and never smoker lung adenocarcinoma patients from Milan, Italy.

ABSTRACT: Alteration of gene expression profile of target organs may signal exposure of that organ to toxic chemicals. We analyzed the transcriptome of the non-involved lung tissue, excised from 176 surgically treated lung adenocarcinoma patients, to identify genes whose expression levels were altered by individual habit to cigarette smoking. Of 17.097 genes analyzed, 357 resulted to be differentially expressed between never smokers and ever smokers (FDR <0.05). The gene that resulted to be the most significantly differentially expressed was MYO1A (FDR = 6.9 x 10-4 and ever versus never smokers fold change of 1.42). We compared our results with those of five independent datasets and found that more than one third (38.6%) of the transcripts associated with smoking habit in our dataset overlapped with at least one of the other datasets, with 7 genes (KMO, CD1A, SPINK5, TREM2, CYBB, DNASE2B, FGG) resulting significantly differentially expressed between ever and never smokers in all five datasets, with concordant higher expression in ever smokers than in never smokers. Most of the genes that we found significantly differentially expressed between ever and never smokers participate in pathways/networks that are directly or indirectly associated with immunity and inflammation, in particular, the most significantly enriched pathway was that of eicosanoid signaling. Overall, present results further points to the inflammatory condition that characterize the lung tissue of smokers.

ORGANISM(S): Homo sapiens

PROVIDER: GSE123352 | GEO | 2019/10/01

REPOSITORIES: GEO

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