Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing (RNA-seq) analysis of leaf and root of Col grown on 1/2 MS with or without PS-SO3H or PS-NH2 nanoplastics treatment

ABSTRACT: The goals of this study are to find the differential expression genes after PS-SO3H or PS-NH2 nanoplastics treatment, to explain why the PS-SO3H or PS-NH2 nanoplastics have inhibited the root growth.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE123369 | GEO | 2018/12/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA sequencing (RNA-seq) analysis of leaf and root of Col grown on 1/2 MS with or without PS-SO3H or PS-NH2 nanoplastics treatment

Project description:RNA sequencing (RNA-seq) analysis of leaf and root of Col grown on 1/2 MS with or without PS-SO3H or PS-NH2 nanoplastics treatment

| PRJNA508516 | ENA

Protein corona formation on nanoplastics: polystyrene and PVC

Project description:In this study, the proteins forming the protein corona on PS and PVC were investigated after 5 min, 1 h, 6 h, or 24 h incubation of the nanoplastics with human plasma. TMT-based untargeted proteomics were applied to identify the proteins forming the protein corona.

2024-10-25 | PXD032162 | Pride

Heterogeneity effects of nanoplastics and lead on zebrafish intestine identified by single-cell sequencing

Project description:Plastic particles in water environment can adsorb heavy metals, leading to combined toxicity to aquatic organisms. However, current conclusions are mostly obtained based on cell population-average responses. Heterogeneity effects among cell populations in aquatic organisms remain unclear. This study analyzed the heterogeneity effects of 200 μg/L 100 nm polystyrene nanoplastics (PS-NPs), 50 μg/L lead (Pb), and their combined exposures on zebrafish intestine cells by single-cell RNA sequencing.A total of 38640 cells in the zebrafish intestine was obtained and identified as seven cell populations, including enterocytes, macrophages, neutrophils, B cells, T cells, enteroendocrine cells, and goblet cells.Co-exposure of PS-NPs and Pb caused similar transcriptome profiles with PS-NPs exposure in macrophages, which changed immunological recognition processes. The Pb exposure influenced the macrophages by direct cytotoxicity. However, the Pb alone and combined exposures induced similar modes of action in the enterocytes, including the generation of oxidative stress and abnormal lipid metabolism.

2022-02-25 | GSE165888 | GEO

Azospira oryzae PS strain:PS

Project description:Azospira oryzae PS strain:PS Genome sequencing

| PRJNA250450 | ENA

Gut-Lung Microbiota Dynamics in Mice Exposed to Nanoplastics

Project description:The objective was to evaluate the impact of nanoplastics on various niches of the microbiome in a mouse model

2024-10-21 | GSE265766 | GEO

Nanoplastics-16s

Project description:Nanoplastics-16s Raw sequence reads

| PRJNA948176 | ENA

Nanoplastics and sunscreens contained in personal care products induce neurotoxicity in early zebrafish embryos

Project description:Nanoplastics with small particle size and high surface area/volume ratio make them easy to absorb environmental pollutants and affect their bioavailability. In this study, polystyrene nanoplastic beads (PS-NPBs) with a particle size of 100nm and sunscreen butyl methoxydibenzoylmethane (BMDBM) contained in personal care products were chosen as target pollutants to study their developmental toxicity and interactive effects on zebrafish embryo. The exposure period was 2-12 hours post fertilization (hpf). The BMDBM and PS-NPBs significantly upregulated the genes related to antioxidant enzymes and downregulated the gene expression of aromatase and DNA methyltransferases, but the influenced genes were not exactly the same, and the combined exposure reduced the adverse effects on the expression of all genes. With the help of single-cell RNA sequencing technology, the neural mid cells were identified as the target cells of both pollutants, and brain development, head development and notch signaling pathway were the functions they commonly altered. The key genes and functions that are specifically affected by BMDBM and/or PS-NPBs were identified. The BMDBM mainly affect the differentiation and fate of neurons in central nervous system through the regulation of her5, her6, her11, lfng, pax2a and fgfr4. The PS-NPBs regulated the expression of olig2, foxg1a, fzd8b, six3a, rx1, lhx2b, nkx2.1a and sfrp5 to alter nervous system development, retinal development, and stem cell differentiation. The phenotypic responses of zebrafish larvae at 120 hpf were tested, and significant inhibition of locomotor activity was found, indicating that early disrupting effect on central nervous system would have a sustained impact on the behavior of zebrafish.

2020-09-12 | GSE157816 | GEO

Sphaerochaeta sp. PS strain:PS

Project description:Sphaerochaeta sp. PS strain:PS Genome sequencing and assembly

| PRJNA1019525 | ENA

Oral ingestion of polystyrene nanoplastics causes memory impairment and neuroinflammation by microglial uptake

Project description:Nanoplastics are produced by breakdown of plastics in environmental contamination or commercial use for cosmetics or daily expenses. Emerging evidence indicate that ingested nanoplastics with a size smaller than 100 nm have the potential to reach the brain and induces neurotoxicity. However, the potential toxicity of nanoplastics on brain are limited because of difficulties in synthesize of nanoplastics. In present study, we synthesized the fluorescent polystyrene nanoplastics (PSNPs) and examined the toxicity of PSNPs in brain in vivo and in vitro analyses by comparison to IR-813 fluorophore. Synthesized PSNPs were characterized by fluorescence imaging system, scanning electron microscopy, and Fourier-transform infrared spectroscopy. PSNPs were detected in adult mice brain by oral ingestion. In addition, a series of behavioral analyses showed that oral ingestion of PSNPs induced memory impairments. Among brain cells, PSNPs were predominantly internalized in microglia, and uptake of PSNPs induced microglial activation. In addition, the conditioned medium derived from microglia exposed to PSNPs repressed hippocampal neuronal activity. Furthermore, transcriptome analysis showed that PSNPs changed gene expressions in microglia, elevation of neuroinflammation in contrast to suppression of neurotrophic factors. These results indicated that predominant uptake of PSNPs in microglia induced elevation of neuroninflammatory responses whereas suppression of neurotrophic factors that may contribute to the cognitive impairment. Our findings indicate the toxic mechanism and potential detrimental effect of nanoplastic in brain and suggest a potential risk of cognitive impairment by exposure to nanoplastics.

2023-09-24 | GSE243612 | GEO

Drosophila algonquin strain:NH2

Project description:Drosophila algonquin strain:NH2 Raw sequence reads

| PRJNA392359 | ENA