Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [TMCC2]

ABSTRACT: HUDEP-2 cells were lentivirally infected with CRISPRi constructs using a nontargeting guide or guides targeting an enhancer in the TMCC2 locus

ORGANISM(S): Homo sapiens

PROVIDER: GSE123510 | GEO | 2019/03/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-03-30 | GSE115684 | GEO

Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [RNA-seq]

Project description:In vitro cultured CD34+ derived erythroblasts were sorted using surface markers and processed using RNA-seq

2019-03-31 | GSE115678 | GEO

Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [ATAC-seq]

Project description:In vitro cultured CD34+ derived erythroblasts were sorted using surface markers and processed using ATAC-seq

2019-03-31 | GSE115672 | GEO

Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis

Project description:Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis

| PRJNA475760 | ENA

Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [TMCC2]

Project description:Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [TMCC2]

| PRJNA508967 | ENA

Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [RNA-seq]

Project description:Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [RNA-seq]

| PRJNA475757 | ENA

Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [ATAC-seq]

Project description:Integrated epigenomic and transcriptomic profiling of terminal human erythropoiesis [ATAC-seq]

| PRJNA475744 | ENA

A Unique Epigenomic Landscape Defines Human Erythropoiesis.

Project description:Mammalian erythropoiesis yields a highly specialized cell type, the mature erythrocyte, evolved to meet the organismal needs of increased oxygen-carrying capacity. To better understand the regulation of erythropoiesis, we performed genome-wide studies of chromatin accessibility, DNA methylation, and transcriptomics using a recently developed strategy to obtain highly purified populations of primary human erythroid cells. The integration of gene expression, DNA methylation, and chromatin state dynamics reveals that stage-specific gene regulation during erythropoiesis is a stepwise and hierarchical process involving many cis-regulatory elements. Erythroid-specific, nonpromoter sites of chromatin accessibility are linked to erythroid cell phenotypic variation and inherited disease. Comparative analyses of stage-specific chromatin accessibility indicate that there is limited early chromatin priming of erythroid genes during hematopoiesis. The epigenome of terminally differentiating erythroid cells defines a distinct subset of highly specialized cells that are vastly dissimilar from other hematopoietic and nonhematopoietic cell types. These epigenomic and transcriptome data are powerful tools to study human erythropoiesis.

| S-EPMC6863094 | biostudies-literature

Gene expression profiling (RNA-seq) experiment where CRISPRi guides were designed to target the human rs2650492 variant, the promoter of GAPDH, and a non-coding region near TUFM in HEK293t cells

Project description:CRISPRi guides were designed to target genomic regions of interest in HEK293t cells. Four guides were designed per region (see processed file). These regions were an enhancer located in the 3’UTR of SBK1 which harbors the obesity GWAS variant rs2650492, the promoter of GAPDH as a positive control, and a non-coding region near TUFM as a negative control. We also provide transcriptomes for Cas9 transfected cells only. Genes proximal to rs2650492 were assessed for differential expression. This experiment was performed with technical replicates where each replicate is a unique transfection and library preparation.

2021-07-14 | E-MTAB-10460 | biostudies-arrayexpress

iPSC RNA-seq upon CRISPRn or CRISPRi manipulation

Project description:Assess the on- and off-target effects of dox-inducible CRISPR/Cas9 and CRISPRi constructs in a human iPS cell line. Transcript quantification of 3 cell lines, each plus or minus doxycycline and with or without specific single guide RNAs (sgRNAs), with 2 biological replicates each.

2016-01-19 | E-GEOD-76980 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data