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Comparing transcriptomic analysis using QuantSeq and RNA-Seq with Salmon quantification and Tophat2 mapping

ABSTRACT: We have compared a relatively new method for RNA 3’ prime sequencing, QuantSeq with traditional RNA-Seq which captures reads from the entire RNA-transcript. In addition, we have compared read quantification using traditional base-to-base mapping (Tophat2) with the new pseudoalignment method Salmon. We have performed this experiment on peripheral blood mononuclear cells (PBMC) stimulated with Poly (I:C), a viral mimic that induces innate antiviral responses.

ORGANISM(S): Homo sapiens

PROVIDER: GSE123523 | GEO | 2019/12/29

REPOSITORIES: GEO

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