Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ultracentifugation and nanoscale deterministic lateral displacement (nanoDLD) of samples for exRNA analysis

ABSTRACT: An integrative analysis of human biofluid data in the exRNA Atlas revealed the existence of distinct extracellular RNA cargo types. To gain further insight on the biological nature of these cargo types, we correlated exRNA Atlas cargo profiles with a variety of other RNA-seq profiles. This study focuses on those samples obtained via ultracentrifugation and nanoscale deterministic lateral displacement (nanoDLD).

ORGANISM(S): Homo sapiens

PROVIDER: GSE123736 | GEO | 2018/12/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Lipoprotein Particle (LPP) exRNA Profiles Isolated from Plasma

Project description:An integrative analysis of human biofluid data in the exRNA Atlas revealed the existence of distinct extracellular RNA cargo types. To gain further insight on the biological nature of these cargo types, we correlated exRNA Atlas cargo profiles with a variety of other RNA-seq profiles. This study focuses on lipoprotein particle (LPP) exRNA profiles obtained via sequential density ultracentrifugation (SD-UC) and fast protein liquid chromatography (FPLC).

2018-12-20 | GSE124131 | GEO

Physically Isolated OptiPrep (TM) Fractions for exRNA Analysis

Project description:An integrative analysis of human biofluid data in the exRNA Atlas revealed the existence of distinct extracellular RNA cargo types. In order to detect differences in density between cargo types, cushioned density gradient ultracentrifugation (C-DGUC) of serum and plasma was performed using OptiPrem (TM) density gradient.

2018-12-15 | GSE123864 | GEO

Assessment of RNA Isolation Kit Biases Toward Preferential Detection of exRNA Cargo Types

Project description:An integrative analysis of human biofluid data in the exRNA Atlas revealed the existence of distinct extracellular RNA cargo types. To determine whether different RNA isolation kits biased detection of certain exRNA cargo types, an integrative analysis was performed using pooled plasma and serum samples, where 10 different RNA isolation kits were applied.

2018-12-15 | GSE123865 | GEO

Ultracentifugation and nanoscale deterministic lateral displacement (nanoDLD) of samples for exRNA analysis

Project description:Ultracentifugation and nanoscale deterministic lateral displacement (nanoDLD) of samples for exRNA analysis

| PRJNA509666 | ENA

ExRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types Present Across Human Biofluids

Project description:ExRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types Present Across Human Biofluids

| PRJNA510084 | ENA

exRNA reference profiling of 13 individuals addressing the effect of biofluid (serum vs. EDTA plasma), individual, gender, fasting and female hormonal cycle

Project description:Circulating extracellular RNAs (exRNAs) have the potential to serve as biomarkers for a wide range of medical conditions. However, limitations in existing exRNA isolation methods and a lack of knowledge on parameters affecting exRNA variability in human samples may hinder their successful discovery and clinical implementation. Using novel combinations of denaturants, reducing agents, proteolysis and revised organic extraction, we developed an automated, high-throughput approach for recovery of exRNAs and extracellular DNA (exDNA) from the same biofluid sample. We applied the method to characterize exRNAs from 312 plasma and serum samples collected from thirteen healthy volunteers at twelve time points over a two-month period.

2018-05-04 | GSE113994 | GEO

AGO2 Plasma exRNA Profiles

Project description:The Michael J. Fox Foundation (MJFF) collected AGO2 exRNA samples for distribution to interested individuals.

2018-12-22 | GSE124269 | GEO

AGO2 Plasma exRNA Profiles

Project description:AGO2 Plasma exRNA Profiles

| PRJNA511445 | ENA

Comparability of the small RNA secretome across human biofluids concomitantly collected from healthy adults

Project description:Small extracellular vesicles (sEV) are nano-sized (40-150 nm), membrane-encapsulated vesicles that are released by malignant or pathologic and non-pathologic cells into the extracellular space and function as intercellular signaling vectors through the horizontal transfer of biologic molecules, including microRNA (miRNA) and other small non-coding RNA (ncRNA), that can alter the phenotype of recipient cells. sEV are present in essentially all extracellular biofluids, including serum, urine and saliva, and offer a new avenue for discovery and development of novel biomarkers of various disease states and exposures. The objective of this study was to determine the similarities and differences between sEV ncRNA derived from saliva, serum and urine, as well as cell-free ncRNA from serum. Saliva, urine and serum were concomitantly collected from 4 healthy donors, and sEV were isolated from each respective biofluid, along with cfRNA from serum. sEVs were isolated from the respective biofluids via differential ultracentrifugation. Small RNA-sequencing was performed on each sample, and cluster analysis was performed based on ncRNA profiles. While some similarities existed in terms of sEV ncRNA cargo across biofluids, there are also notable differences in ncRNA class and ncRNA secretion, with each sEVs in each biofluid bearing a unique ncRNA profile. We conclude that sEV ncRNA cargo varies according to biofluid, so thus should be carefully selected and interpreted when designing translational or epidemiological studies.

2020-02-25 | GSE122621 | GEO

Assessment of RNA Isolation Kit Biases Toward Preferential Detection of exRNA Cargo Types

Project description:Assessment of RNA Isolation Kit Biases Toward Preferential Detection of exRNA Cargo Types

| PRJNA510086 | ENA