Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Raw RNA-seq data of HSV-1-infected human cells (HFF and MRC5)

ABSTRACT: We report transcriptomic data from HSV-1-infected human cells (HFF and MRC5) Herpes simplex virus type I (HSV-1) is a common human pathogen causing cold sores, and in rare cases, severe keratitis and encephalitis. Mouse models are commonly used to study pathogenesis of HSV-1 infection due to the neurotropic properties of HSV make it hard to reach information from infected humans, but mice are not a natural host for this virus. Therefore, it is important to have insights into transcriptional regulation in human cell cultures, which gave us more information before we interpret experimental results from humans and mouse models. Herein, we provide overall transcriptomic data from two HSV-1infected cells, HFF and MRC5. We found that these two human cells downregulated many genes in an antiviral pathway characterized by interferon-stimulated genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE124118 | GEO | 2021/12/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Raw RNA-seq data of HSV-1-infected human cells (HFF and MRC5)

Project description:Raw RNA-seq data of HSV-1-infected human cells (HFF and MRC5)

| PRJNA510802 | ENA

Transcription start site profiling of HSV-1 infected cells using cRNA-seq and dRNA-seq

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Sequencing libraries were prepared using the cRNA-seq and dRNA-seq protocol from the indicated time points of infection.

2020-04-07 | GSE128323 | GEO

ATAC-seq on HSV-1 infected primary human fibroblasts (time course)

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. ATAC-seq libraries were prepared starting with 50,000 cells per condition following the protocol described by Buenrostro et al., Nature Methods 2013

2018-03-08 | GSE100611 | GEO

Subcellular RNA fractions of HSV-1 infected primary human fibroblasts

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Subcellular RNA fractions including total RNA, cytoplasmic RNA, nucleoplasmic RNA and chromatin-associated RNA were prepared and subjected to RNA-seq

2018-03-08 | GSE100576 | GEO

Wide-spread disruption of transcription termination in HSV-1 infection: Next-generation sequencing of translational activityd by ribosome profiling

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Ribosome profiling was performed at various times during infection with minor modification to the protocol described in Stern-Ginossar N et al., Science 2012

2015-05-26 | GSE60040 | GEO

Subcellular RNA fractions of HSV-1 infected primary human fibroblasts including infection with delta-Vhs mutant

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 or its vhs null mutant at a multiplicity of infection (MOI) of 10. Subcellular RNA fractions including total RNA, cytoplasmic RNA, nucleoplasmic RNA and chromatin-associated RNA were prepared and subjected to RNA-seq.

2020-10-26 | GSE129582 | GEO

Subcellular RNA fractions of primary human fibroblasts infected with the HSV-1 delta-ICP27 mutant

Project description:Primary human foreskin fibroblasts (HFF) were infected with the ICP27 null mutant of herpes simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Subcellular RNA fractions including total RNA, cytoplasmic RNA, nucleoplasmic RNA and chromatin-associated RNA were prepared and subjected to RNA-seq

2020-04-09 | GSE128880 | GEO

Chromatin-associated RNA of HSV-1 infected primary human fibroblasts including infection with vhs mutants

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type herpes simplex virus 1 (HSV-1) strain 17 or its vhs null mutant, or with BAC-derived viruses expressing either a wild-type or catalytically inactive vhs with the D195N mutation at a multiplicity of infection (MOI) of 10. Chromatin-associated RNA was prepared and subjected to RNA-seq.

2020-10-26 | GSE140068 | GEO

Wide-spread disruption of transcription termination in HSV-1 infection: Next-generation sequencing of translational activityd by ribosome profiling

2015-05-26 | E-GEOD-60040 | biostudies-arrayexpress

RNA-Seq of influenza A/Puerto Rico/8/1934 (H1N1) virus infected MRC5, WI-38 VA-13, A549 and HEK293FT human cells

Project description:To study genes involved in cellular response to Influenza A virus infection, human cells MRC5, WI-38 VA-13, A549 and HEK293FT were infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. RNA-seq analysis of infected cell lines after 48 h was performed on an Illumina NextSeq 500 platform. The same mock-infected cells were used as controls. PolyA RNA-enriched fraction was used for constructing of cDNA libraries. Differential expressed genes were identified using R package DESeq2.

2020-11-23 | E-MTAB-9511 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data