Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Liver, Adipose Tissue, and Skeletal Muscle from adult male offspring of maternal nutrient restriction in mice

ABSTRACT: Pregnant CD-1 mice were fed control (ad libitum) and 70% total food (g) (MNR) diets from E6.5-birth. At birth, litters with 11-15 pups were cross-fostered to ad libitum-fed mothers. At weaning, males were kept for analysis and fed ad libitum until 6 months when they were euthanized by CO2 necropsis for quadriceps femoris, right medial liver lobe and perigonadal adipose tissue. Tissues were frozen in liquid nitrogen until total RNA isolations were done on whole-tissue homogenate. Libraries were made with polyA enrichment and sequenced. Differential expression between maternal nutrition or glucose tolerance groups (glucose intolerant MNR, tolerant MNR, MNR with a high fasting blood glucose [IFBG] and controls).

ORGANISM(S): Mus musculus

PROVIDER: GSE124303 | GEO | 2019/12/19

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Hepatic gene expression in male fetuses from maternal nutrient restriction in mice

Project description:The aim of this study was to identify fetal adaptations to gene expression in our mouse model of intrauterine growth restriction (IUGR). Starting at E6.5, pregnant CD-1 mice received either control (ad libitum) or 70% of ad libitum food (maternal nutrient restriction [MNR]). At E18.5, fetal right liver lobes were collected from 2 pups/litter in litters with 11-15 pups. Frozen liver was homogenized for RNA isolations. Isolated RNA was sequenced on a HiSeq2500 with an average read depth of 23M reads and a range of 17-31M reads per sample. Reads were aligned to the mm9 transcriptome with Bowtie2. Normalization and differential expression was done with DESeq2, ALDex2 and EdgeR, and genes were considered in the study if they were consistent in 2 or more tools (FDR <0.1).

2019-12-17 | GSE124006 | GEO

Transcription profiling of mouse liver from animals that vary in adiposity and insulin sensitivity

Project description:Adipose tissue gene expression was profiled from perigonadal adipose tissue of mice whose body mass and insulin sensitivity varied as a function of diet, gender, monogenic mutations and insulin sensitizing therapies. Mice were 22-24 weeks of age and sacrificed between 2-3 hours into the light portion of a 12/12 dark-light cycle. The goal was to identify transcripts whose adipose tissue expression is correlated with adiposity, insulin sensitivity and other measures of metabolic function. Experiment Overall Design: Mice on the C57BL/6J strain were housed in groups of 3-5 and fed ad libitum. 2-4 days prior to collection of adipose tissue fasting blood glucose and serum insulin concentrations were measured. Mice were sacrificed 2-3 hours into the light cycle. RNA was extracted from perigonadal adipose tissue and used to generate labed cRNA for hybridization to Affymetrix Mu74Av2 microarrays.

2008-11-13 | E-GEOD-8831 | biostudies-arrayexpress

Expression of obese mice subjected to diet induced weight loss for 60 days

Project description:We used microarrays to study the gene expression dynamics during caloric restricted weight loss in obese mice after feeded with high fat diet. C57BL/6J male mice were placed on a high fat diet at 10-11 weeks of age in individual cages and kept on the diet until they reached ~ 41 g. After all the baseline (41g) measurement were made, each mouse was fed 75% of their ad libitum food intake in two divided daily "meals". The high fat diet was maintained. Mice were sacrificed after 0, 1, 3, 7, 14, 21 or 42 day of caloric restriction. Hypothalamus, liver, abdominal subcutaneous adipose, mesenteric adipose, perirenal adipose and perigonadal adipose tissues were collected for RNA extraction and hybridization on Affymetrix microarrays.

2012-12-30 | E-GEOD-30534 | biostudies-arrayexpress

Effect of maternal nutrient reduction (MNR) during pregnancy on fetal liver gene expression in baboons (Papio anubis).

Project description:The objective was to determine changes in gene expression in baboon fetal livers at 90, 120, 140 and 165 days gestation (dG) due to reduction of feed consumed by dams. Control dams were fed ad libitum throughout the study, while 30% feed reduction (based on feed consumed by controls at the same gestation stage and adujsted for body weight) started at 30 dG for MNR dams. RNA-seq data was analyzed to identify differentially expressed genes and transcript variants across four time points.

2024-08-11 | GSE211085 | GEO

Effects of ursolic acid and tomatidine on skeletal muscle mRNA expression in aged mice.

Project description:For additional details see Ebert et al, Identification and Small Molecule Inhibition of an ATF4-dependent Pathway to Age-related Skeletal Muscle Weakness and Atrophy. Weight-matched cohorts of 22-month-old male C57BL/6 mice were provided ad libitum access to standard chow (control) or standard chow supplemented with 0.27% ursolic acid (UA) or 0.05% tomatidine (TM) for 2 months. After the 2 month treatment period, quadriceps femoris muscles were harvested. mRNA levels in muscles harvested from ursolic acid or tomatidine fed mice were normalized to levels in muscles fed control diet.

2015-09-03 | E-GEOD-72651 | biostudies-arrayexpress

Liver transcriptomic response to fasting, high glucose or a high fructose challenges in wild-type C57Bl/6J male mice

Project description:Microarrays experiment was performed to detail the global programme of gene expression in liver of WT male mice in response to fasting (for 24h) or challenged with high glucose or high fructose compared to mice fed ad libitum.

2017-10-13 | GSE92502 | GEO

Global hepatic transcript data from LRH-1 hep+/+ and LRH-1 hep-/- livers

Project description:Transcript data from LRH-1 hep+/+ and LRH-1 hep-/- livers from mice fed ad libitum and sacrificed at 7 am We used microarrays to detail the global programme of gene expression underlying hepatic function in ad libitum fed mice.

2014-07-11 | GSE59305 | GEO

Global hepatic transcript data from LRH-1 WT and LRH-1 K289R livers

Project description:Transcript data from LRH-1 WT and LRH-1 K289R livers from mice fed ad libitum and sacrificed at 7 am We used microarrays to detail the global programme of gene expression underlying hepatic function in ad libitum fed mice.

2014-07-11 | GSE59304 | GEO

Global hepatic transcript data from LRH-1 WT and LRH-1 K289R jejunums

Project description:Transcript data from LRH-1 WT and LRH-1 K289R jejunums from mice fed ad libitum and sacrificed at 7 am We used microarrays to detail the global programme of gene expression underlying jejunal function in ad libitum fed mice.

2014-07-11 | GSE59303 | GEO

Mouse gestational protein restriction: Newborn offspring liver and hindleg muscle

Project description:Gestational protein restriction is a model for low birth size. We hypothesized that taurine supplementation would protect against changes in newborn liver and muscle caused by a maternal low protein diet. Pregnant mouse dams were subjected to different diet schemes from day 1 of pregnancy until birth. Pups were killed following birth and liver and hindleg skeletal muscle taken out and frozen at -80C until analysis. Diet schemes: Normal Protein (20% casein; NP), Normal Protein + taurine (1% taurine supplementation in water ad libitum; NP+tau), Low Protein (8% casein; LP) and LP+tau The liver and muscle samples were normalized separately.

2009-11-07 | E-GEOD-12730 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data