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MRNA expression in ttcsp1 deletion mutant and wild type Thermus thermophilus HB8 strains grown on a rich medium

ABSTRACT: We observed the expression profile of the total mRNA of ttcsp1 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Keywords: temperature comparison

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE12436 | GEO | 2009/07/31

SECONDARY ACCESSION(S): PRJNA112987

REPOSITORIES: GEO

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Transcription profiling of Thermus thermophilus HB8 wild-type strain grown on a rich medium

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Experiment Overall Design: wild-type T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.3 ml) were inoculated into 0.15 liter of the same medium and then cultivated at 70oC. At the Abs600=0.8 (0min), cold medium was added and the temperature were shifted down to 45oC. Cells were collected at 0min and 30min after temperature shift, and then crude RNA was extracted. In order to compare mRNA expression in thewild-type cell between 45oC and 70oC, the expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2009-01-09 | E-GEOD-12437 | biostudies-arrayexpress

mRNA expression in ttcsp1 deletion mutant and wild type Thermus thermophilus HB8 strains grown on a rich medium

Project description:We observed the expression profile of the total mRNA of ttcsp1 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TR) medium at 45oC compared with that of 70oC. Keywords: temperature comparison Wild-type and ttcsp1 mutant T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.3 ml) were inoculated into 0.15 liter of the same medium and then cultivated at 70oC. At the Abs600=0.8 (0min), cold medium was added and the temperature were shifted down to 45oC. Cells were collected at 0min and 30min after temperature shift, and then crude RNA was extracted. In order to compare mRNA expression in the ttcsp1 mutant between 45oC and 70oC, the expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC. Keywords: time course

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Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70°C. Keywords: time course

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

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Comparative expression analysis between sigE (TTHB211) deletion mutant and wild-type of T. thermophilus HB8

Project description:We observed the expression profile of the total mRNA of sigE (TTHB211) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC compared with that of wild type. Keywords: cell type comparison

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