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A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE124486 | GEO | 2018/12/30

REPOSITORIES: GEO

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A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [Bisulfite-seq]

Project description:We found SUVH-SDJ complex occupies promoter methylated genes and promotes the expression of these genes.

2018-12-27 | GSE124376 | GEO

A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [ChIP-seq]

Project description:We found SUVH-SDJ complex occupies promoter methylated genes and promotes the expression of these genes.

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A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [RNA-seq]

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A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes

Project description:A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes

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A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [RNA-seq]

Project description:A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [RNA-seq]

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A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [Bisulfite-seq]

Project description:A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [Bisulfite-seq]

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A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [ChIP-seq]

Project description:A methylated-DNA-binding complex required for plant development mediates transcriptional activation of promoter methylated genes [ChIP-seq]

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SMAD 8 binding to mice Msx1 basal promoter is required for transcriptional activation.

Project description:The Msx1 gene in mice has been proven to be induced by BMP (bone morphogenetic protein) proteins, and three binding sites for SMAD, an intracellular BMP signalling transducer, have already been identified in its promoter. Gel shift analyses were performed and they demonstrated that the consensus found very near the transcription start site, a region designed BP (basal promoter), is functional for binding nuclear proteins from 10.5, 11.5 and 13.5 dpc (days post-coitum) embryos. Notably, this binding occurs only when the SMAD-binding consensus sequence is maintained, suggesting that it is required for the formation of a protein complex over BP. Binding of purified SMAD 1 and SMAD 4 as well as supershift assay with SMAD 1/SMAD 5/SMAD 8 antibody proved that a SMAD protein is present in this complex. Transfection assays in cell cultures with fragments from BP driving the expression of luciferase confirmed that only in the presence of the SMAD consensus site is Msx1 expression activated. A proteomic analysis of the complex components after immunoprecipitation identified several proteins necessary to activate transcription including SMAD 8. Our results suggest that BMP2/BMP4 signalling through SMAD 8 is required for transcriptional activation of the mouse Msx1 gene.

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The non-methylated DNA-binding function of Kaiso is not required in early Xenopus laevis development.

Project description:Mammalian forms of the transcription repressor, Kaiso, can reportedly bind methylated DNA and non-methylated CTGCNA motifs. Here we compare the DNA-binding properties of Kaiso from frog, fish and chicken and demonstrate that only the methyl-CpG-binding function of Kaiso is evolutionarily conserved. We present several independent experimental lines of evidence that the phenotypic abnormalities associated with xKaiso-depleted Xenopus laevis embryos are independent of the putative CTGCNA-dependent DNA-binding function of xKaiso. Our analysis suggests that xKaiso does not play a role in the regulation of either xWnt11 or Siamois, key signalling molecules in the Wnt pathway during X. laevis gastrulation. The major phenotypic defects associated with xKaiso depletion are premature transcription activation before the mid-blastula transition and concomitant activation of a p53-dependent cell-death pathway.

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MBD2a-NuRD binds to the methylated γ-globin gene promoter and uniquely forms a complex required for silencing of HbF expression.

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