Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ImmGen ULI: Male-Female Immune Differences

ABSTRACT: Primary RNA Seq data for 11 diverse immunocyte populations from male and female mice of varying ages stimulated with different dose of IFN and sequenced using ImmGen's standard ultra-low input RNA-seq pipeline

ORGANISM(S): Mus musculus

PROVIDER: GSE124829 | GEO | 2019/01/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

ImmGen ULI RNA-seq data

Project description:Primary RNAseq data from carefully sorted immunocyte populations, sequenced using ImmGen's SOP for 'ultra-low-input' population RNAseq (typically 500 to 1,000 cells) performed by Smartseq2.

2019-02-27 | GSE127267 | GEO

ImmGen ULI: Systemwide RNA-seq profiles (#1)

Project description:Primary RNAseq data for 127 highly purified immunocyte populations representing all lineages and several differentiation cascades, profiled using the ImmGen ULI pipeline.

2018-01-13 | GSE109125 | GEO

ImmGen ULI: Deep RNA-seq data

Project description:Primary RNAseq data for five highly purified immunocyte populations (splenic NK, Follicular B, Naïve CD4+ abT, gdT cells and peritoneal macrophages) profiled to unusual depth as multiple biological replicates, using the ImmGen ULI pipeline.

2018-11-16 | GSE122597 | GEO

ImmGen ULI: Male-Female Immune Differences

Project description:ImmGen ULI: Male-Female Immune Differences

| PRJNA513879 | ENA

ImmGen ULI: OpenSource Mononuclear Phagocytes Project

Project description:Primary RNASeq data for progenitor, resident, and stimulated (C.alb, LPS, injury, APAP+ starved overnight and pIC) mononuclear phagocytes from fourteen organs.

2018-11-03 | GSE122108 | GEO

ImmGen ULI RNA-seq data

Project description:ImmGen ULI RNA-seq data

| PRJNA524634 | ENA

ImmGen ULI: Deep RNA-seq data

Project description:ImmGen ULI: Deep RNA-seq data

| PRJNA505693 | ENA

ImmGen ULI: OpenSource Mononuclear Phagocytes Project

Project description:ImmGen ULI: OpenSource Mononuclear Phagocytes Project

| PRJNA503607 | ENA

microRNA profiling of female and male mouse pituitary gland across pubertal transition

Project description:The pituitary gland exhibits sex differences in its function. Diseases associated with dysregulation of the pituitary are also sex-biased in prevalence. Previous qPCR profiling of puberty-related genes in the pituitary gland revealed increasingly sex-biased expression of genes profiled across pubertal transition. Here, we performed small RNA-seq on total RNA extracted from C57BL/6J mouse pituitary gland of both sexes mice at 4 ages spanning pubertal transition (postnatal days 12, 22, 27, 32) (6 replicates per sex at each age) to examine microRNA (miRNA) regulation of sex differences in gene expression observed. Total RNA was sent to SickKids TCAG core to construct small RNA-seq libraries using NEBNext Small RNA Library Prep Kit according to manufacterer’s protocol. Resulting single-end libraries were sequenced at TCAG core on the Illumina HiSeq 2500 v4 flow cell with SR50 bp. Data obtained was processed using miRDeep2 pipeline to align small RNA-seq reads to the mouse genome (mm10) and to filter for miRNAs.

2022-10-12 | E-MTAB-9460 | biostudies-arrayexpress

3'-UTR-seq of female and male mouse pituitary gland across pubertal transition

Project description:The pituitary gland exhibits sex differences in its function. Diseases associated with dysregulation of the pituitary are also sex-biased in prevalence. Previous qPCR profiling of puberty-related genes in the pituitary gland revealed increasingly sex-biased expression of genes profiled across pubertal transition. Here, we performed 3’-UTR-seq on pituitary gland from both sexes of C57BL/6J mice at 5 ages spanning pubertal transition (postnatal days 12, 22, 27, 32, 37) (6 replicates per sex at each age) to examine genome-wide sex-biased trends in gene expression and potential regulatory mechanisms of these sex differences. QuantSeq 3’mRNA-seq libraries were constructed from total RNA using an automated method with Agilent NGS Workstation. Resulting single-end libraries were sequenced at SickKids TCAG core on the Illumina v4 flow cell with SR50 bp cycles extended to 68 bp. A customized pipeline was developed and used for analysis of reads obtained (see paper for details). Processed reads were mapped to mouse genome (mm10).

2022-10-12 | E-MTAB-9459 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data