Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mini-INTACT reveals social isolation-induced epigenetic changes in Drosophila dopaminergic neurons

ABSTRACT: We introduce a more sensitive method for purifying specific cell-types from small numbers of fly heads for epigenomic analysis: a method we call ‘mini-INTACT’. We modified the INTACT method in Drosophila [Henry G. L. et al., Nucleic Acids Research, 2012, 1–14] to enable purification of genetically tagged rare cell types from Drosophila brain requiring ~50-100 fold less material. We used male fruit flies as a model to examine the effects of social-isolation vs. social-enrichment on the epigenome and transcriptome of dopaminergic neurons defined by expression of the TH-GAL4 driver. Using ChIP-seq on mini-INTACT purified nuclei, RNA-seq and bioinformatic analysis, we identify regulation of the transcriptome and behavior by Activity Related Genes who’s expression is induced by social enrichment.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE126186 | GEO | 2019/02/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

mini-INTACT reveals social isolation-induced epigenetic changes in Drosophila dopaminergic neurons

Project description:mini-INTACT reveals social isolation-induced epigenetic changes in Drosophila dopaminergic neurons

| PRJNA521256 | ENA

Trypanosoma cruzi mini-exon based mRNA amplification technique

Project description:We have developed a method of mRNA amplification based on T7 polymerase, where T7 promoter is attached to a oligonucleotide containing the mini-exon sequence, which will hybridize with cDNA molecules containing the anti-ME sequence

2017-07-31 | GSE94766 | GEO

TTV-like mini virus

Project description:TTV-like mini virus Raw sequence reads

| PRJNA347278 | ENA

Infection characteristics and transcriptomics of African swine fever virus in Bama mini-pigs

Project description:To analyse the host responses of Bama mini-pigs to ASFV infection, we chose the spleen and inguinal lymph nodes from Bama mini-pigs infected with ASFV at a dose of 103 HAD50 and the mock group for transcriptomic analysis.

2022-10-14 | GSE215197 | GEO

Construction of Mini-Chemostats for High-throughput Strain Characterization

Project description:We developed a mini-chemostat system with 16 reactors, each at a working volume of 40 ml. Sensors measure dissolved oxygen in the reactor, while OD600 is measured in the outflow. We further developed a CO2 and pH sensor array that can be plugged in to the outflow of the reactors. The system was used to characterize yeast physiology at four metabolically different conditions: limitations of glucose, both aerobic and anaerobic, nitrogen, and ethanol. The physiology of yeast cells grown at the four different conditions in the mini-chemostat (MC) system was compared with yeast cells grown in a DASGIP 1L system using RNAseq analysis

2019-02-01 | GSE120188 | GEO

Spatiotemporally resolved ex vivo colorectal cancer development in engineered mini-colons (scRNA-Seq)

Project description:Here we developed topobiologically complex mini-colons able to undergo tumorigenesis ex vivo by integrating microfabrication, optogenetic, and tissue engineering approaches. With this system, tumorigenic transformation can be spatiotemporally controlled by directing oncogenic activation through blue-light exposure, and emerging colon tumors can be tracked in real-time with single-cell resolution for several weeks without breaking the culture. These induced mini-colons display rich intra- and inter-tumoral diversity and recapitulate key pathophysiological hallmarks displayed by colorectal tumors in vivo. By fine-tuning cell-intrinsic and extrinsic parameters, mini-colons can be leveraged to unveil tumorigenic determinants, including dietary patterns, microbiota-derived metabolites, and pharmacological therapies.

2024-02-24 | GSE221162 | GEO

Sus scrofa scrofa breed:Mini-pig

Project description:Sus scrofa scrofa breed:Mini-pig Raw sequence reads

| PRJNA374593 | ENA

Reconstruction of Dynamic Mammary Mini Gland in vitro for Normal Physiology and Oncogenesis

Project description:Organoid culture has been extensively exploited for normal tissue reconstruction and disease modeling, however, it is still challenging to establish physiologically relevant architecture, size and functions in homeostasis. Here, we describe the development of a long-term adult stem cell derived mammary mini-gland culture system that supports robust 3D outgrowths recapitulating the morphology, cellular context, transcriptional heterogeneity of the normal mammary gland. The self-organization ability of stem cells and the stability of the outgrowths were determined by a coordinated combination of extracellular matrix, environmental signalings and dynamic physiological cycles. These mini glands are hormone responsive and reproduce the entire postnatal mammary development including puberty, estrous cycle, lactation and involution. Most intriguingly, these mini glands maintained the mammary stem cells and represented the fate transition from embryonic bipotency to postnatal unipotency in lineage tracing assays. In addition, induced oncogene expression in the mini glands drove the clinically relevant cancer initiation that can be monitored chronologically. Together, this study provides an experimental system fostering a dynamic organ miniature that can be studied chronologically and spatially for physiologically relevant biological processes with complexity.

2023-08-20 | GSE211527 | GEO

Leptospira borgpetersenii serovar Mini str. 201000851

Project description:Leptospira borgpetersenii serovar Mini str. 201000851 Genome sequencing and assembly

| PRJNA74175 | ENA

Spatiotemporally resolved ex vivo colorectal cancer development in engineered mini-colons (RNA-Seq Gpx2 KD)

2024-02-24 | GSE246568 | GEO