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Accurate detection of m6A RNA modifications in native RNA sequences [Yeast]

ABSTRACT: The field of epitranscriptomics has undergone an enormous expansion in the last few years; however, a major limitation is the lack of generic methods to map RNA modifications transcriptome-wide. Here we show that using Oxford Nanopore Technologies, N6-methyladenosine (m6A) RNA modifications can be detected with high accuracy, in the form of systematic errors and decreased base-calling qualities. Our results open new avenues to investigate the universe of RNA modifications with single nucleotide resolution, in individual RNA molecules.

ORGANISM(S): Saccharomyces cerevisiae SK1

PROVIDER: GSE126213 | GEO | 2019/09/09

REPOSITORIES: GEO

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