Transcriptomics

Dataset Information

0

Non-supervised hierarchical clustering of gene expression data


ABSTRACT: Despite the frequent detection of circulating tumor antigen-specific T cells, either spontaneously or following active immunization or adoptive transfer, immune-mediated cancer regression occurs only in the minority of patients. One theoretical rate-limiting step is whether effector T cells successfully migrate into metastatic tumor sites. Affymetrix gene expression profiling performed on a series of metastatic melanoma biopsies revealed a major segregation of samples based on the presence or absence of T cell-associated transcripts. The presence of lymphocytes correlated with the expression of defined chemokine genes. A subset of 6 chemokines (CCL2, CCL3, CCL4, CCL5, CXCL9, and CXCL10) was confirmed by protein array and/or quantitative RT-PCR to be preferentially expressed in tumors that contained T cells. Corresponding chemokine receptors were found to be upregulated on human CD8+ effector T cells, and transwell migration assays confirmed the ability of each of these chemokines to promote migration of CD8+ effector cells in vitro. Screening by chemokine protein array identified a subset of melanoma cell lines produced a similar broad array of chemokines. These melanoma cells more effectively recruited human CD8+ effector T cells when implanted as xenografts in NOD/scid mice in vivo. Chemokine blockade with specific antibodies inhibited migration of CD8+ T cells. Our results suggest that lack of critical chemokines in a subset of melanoma metastases may limit the migration of activated T cells, which in turn could limit the effectiveness of anti-tumor immunity. Keywords: Tumor sample microarray

ORGANISM(S): Homo sapiens

PROVIDER: GSE12627 | GEO | 2008/08/30

SECONDARY ACCESSION(S): PRJNA112797

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2008-10-25 | E-GEOD-12627 | biostudies-arrayexpress
2015-08-13 | GSE49632 | GEO
2024-11-05 | GSE270960 | GEO
2024-11-05 | GSE271338 | GEO
2008-12-30 | E-GEOD-12027 | biostudies-arrayexpress
2012-07-19 | E-GEOD-39508 | biostudies-arrayexpress
2008-12-31 | GSE12027 | GEO
2023-05-24 | PXD034033 | JPOST Repository
2023-06-16 | MSV000092193 | MassIVE
2015-05-05 | E-GEOD-65627 | biostudies-arrayexpress