Project description:To analyze the effects of PAC1 on acetylation of H3K27 in exhausted CD8+ T cells, we isolated CD8+ T cells from spleen of wild-type or PAC1-/- mice post LCMV Cl13 infection and performed ChIP-seq with anti-H3K27-ac.
Project description:We identified that Gsdmc gene is important for pancreatic cancer in tumorigenesis and metastasis. Here we used KPC mice (LSL-KrasG12D/+; p53f/f; Pdx1-Cre)-derived cancer cells, KPC cells in short, to check the effects of Gsdmc knockdown on primary tumor growth and metastasis. The results suggested that Gsdmc knockdown inhibited the expression of key genes in multiple pathways, including cell migration, epithelial-mesenchymal transition, immune cell recruitment, and so on. The data deposited here provides the transcriptomic alteration of KPC cells after Gsdmc knockdown with two shRNAs.
