Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Essential functions of the Glucocorticoid Receptor require direct DNA binding

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE126655 | GEO | 2019/12/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Essential functions of the Glucocorticoid Receptor require direct DNA binding [RNA-seq]

Project description:We performed RNA sequencing in Mouse Embryonic Fibroblasts (MEFs) of GR wild-type and GR Zn. Cells were treated with: vehicle (EtOH), Dexamethasone (Dex), Lipopolysaccharide (LPS) or Dex+LPS. We show that GR Zn does not regulate any GR target gene

2019-12-31 | GSE124919 | GEO

Essential functions of the Glucocorticoid Receptor require direct DNA binding [ChIP-seq]

Project description:We performed ChIP-Sequencing for GR in GR wild-type, GR Zn mutant and GR Knock-Out Mouse Embryonic Fibroblasts (MEFs). We show that GR Zn mutant chromatin binding is mostly lost except for some tethered binding sites. Together with the corresponding RNA-Seq data, our results illustrate how GR requires direct DNA binding for activation and repression of target genes.

2019-12-31 | GSE126654 | GEO

Essential functions of the Glucocorticoid Receptor require direct DNA binding

Project description:Essential functions of the Glucocorticoid Receptor require direct DNA binding

| PRJNA522804 | ENA

Anti-inflammatory functions of the glucocorticoid receptor require DNA binding.

Project description:The glucocorticoid receptor is an important immunosuppressive drug target and metabolic regulator that acts as a ligand-gated transcription factor. Generally, GR's anti-inflammatory effects are attributed to the silencing of inflammatory genes, while its adverse effects are ascribed to the upregulation of metabolic targets. GR binding directly to DNA is proposed to activate, whereas GR tethering to pro-inflammatory transcription factors is thought to repress transcription. Using mice with a point mutation in GR's zinc finger, that still tether via protein-protein interactions while being unable to recognize DNA, we demonstrate that DNA binding is essential for both transcriptional activation and repression. Performing ChIP-Seq, RNA-Seq and proteomics under inflammatory conditions, we show that DNA recognition is required for the assembly of a functional co-regulator complex to mediate glucocorticoid responses. Our findings may contribute to the development of safer immunomodulators with fewer side effects.

| S-EPMC7470971 | biostudies-literature

Essential functions of the Glucocorticoid Receptor require direct DNA binding [ChIP-seq]

Project description:Essential functions of the Glucocorticoid Receptor require direct DNA binding [ChIP-seq]

| PRJNA522805 | ENA

Essential functions of the Glucocorticoid Receptor require direct DNA binding [RNA-seq]

Project description:Essential functions of the Glucocorticoid Receptor require direct DNA binding [RNA-seq]

| PRJNA514310 | ENA

Genetic, functional and molecular features of glucocorticoid receptor binding

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-04-01 | E-GEOD-45640 | biostudies-arrayexpress

Androgen and glucocorticoid receptor direct distinct transcriptional programs by receptor-specific and shared DNA binding sites.

Project description:The glucocorticoid (GR) and androgen (AR) receptors execute unique functions in vivo, yet have nearly identical DNA binding specificities. To identify mechanisms that facilitate functional diversification among these transcription factor paralogs, we studied them in an equivalent cellular context. Analysis of chromatin and sequence suggest that divergent binding, and corresponding gene regulation, are driven by different abilities of AR and GR to interact with relatively inaccessible chromatin. Divergent genomic binding patterns can also be the result of subtle differences in DNA binding preference between AR and GR. Furthermore, the sequence composition of large regions (>10 kb) surrounding selectively occupied binding sites differs significantly, indicating a role for the sequence environment in guiding AR and GR to distinct binding sites. The comparison of binding sites that are shared shows that the specificity paradox can also be resolved by differences in the events that occur downstream of receptor binding. Specifically, shared binding sites display receptor-specific enhancer activity, cofactor recruitment and changes in histone modifications. Genomic deletion of shared binding sites demonstrates their contribution to directing receptor-specific gene regulation. Together, these data suggest that differences in genomic occupancy as well as divergence in the events that occur downstream of receptor binding direct functional diversification among transcription factor paralogs.

| S-EPMC8053126 | biostudies-literature

FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-02-05 | E-GEOD-39880 | biostudies-arrayexpress

Genetic, functional and molecular features of glucocorticoid receptor binding

Project description:This SuperSeries is composed of the SubSeries listed below.

2013-04-01 | GSE45640 | GEO