Transcriptomics

Dataset Information

0

RNA sequencing of isogenic BRCA2 haploinsufficient vs. wild-type T-ALL cells


ABSTRACT: We found a high frequency of heterozygous Fanconi-BRCA pathway mutations in pediatric T-ALL. BRCA2 was the most commonly mutated gene. We transduced Cas9-expressing Jurkat cells, which lacked an identifiable BRCA2 mutation, with an integration-defective lentiviral guide RNA expression construct targeting exon 11 of BRCA2 (NM_000059). Single-cell cloning and sequencing analysis revealed two distinct clones harboring monoallelic BRCA2 frameshift mutations, termed clones W4 and W5. Each of these clones was subjected to RNA sequencing analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE126780 | GEO | 2019/02/20

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-09-01 | GSE243142 | GEO
2021-07-26 | MSV000087884 | MassIVE
2012-02-01 | E-GEOD-32124 | biostudies-arrayexpress
2012-02-01 | E-GEOD-32258 | biostudies-arrayexpress
2017-06-02 | PXD006106 | Pride
2021-12-31 | GSE111688 | GEO
2017-01-10 | GSE80685 | GEO
2012-11-23 | E-GEOD-39710 | biostudies-arrayexpress
2015-07-06 | E-GEOD-70003 | biostudies-arrayexpress
2012-02-01 | GSE32258 | GEO