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The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [ChIP1]

ABSTRACT: Genome wide distribution of dBigH1 and dH1 in wild type cells and cells expressing dBigH1

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE127224 | GEO | 2020/02/17

REPOSITORIES: GEO

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The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [ChIP2]

Project description:Genome wide distribution of dBigH1 and dH1 in cells expressing dBigH1ΔED. Genome wide distribution of Rpb3 in wild type S2 cells, cells expressing dBigH1 and cells expressing dBigH1ΔED.

2020-02-17 | GSE127225 | GEO

The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [Affy]

Project description:Expression data from S2 cells wild type and expressing dBigH1

2020-02-17 | GSE127226 | GEO

The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin

Project description:The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin

| PRJNA524369 | ENA

The embryonic linker histone dBigH1 alters the functional state of active chromatin.

Project description:Linker histones H1 are principal chromatin components, whose contribution to the epigenetic regulation of chromatin structure and function is not fully understood. In metazoa, specific linker histones are expressed in the germline, with female-specific H1s being normally retained in the early-embryo. Embryonic H1s are present while the zygotic genome is transcriptionally silent and they are replaced by somatic variants upon activation, suggesting a contribution to transcriptional silencing. Here we directly address this question by ectopically expressing dBigH1 in Drosophila S2 cells, which lack dBigH1. We show that dBigH1 binds across chromatin, replaces somatic dH1 and reduces nucleosome repeat length (NRL). Concomitantly, dBigH1 expression down-regulates gene expression by impairing RNApol II binding and histone acetylation. These effects depend on the acidic N-terminal ED-domain of dBigH1 since a truncated form lacking this domain binds across chromatin and replaces dH1 like full-length dBigH1, but it does not affect NRL either transcription. In vitro reconstitution experiments using Drosophila preblastodermic embryo extracts corroborate these results. Altogether these results suggest that the negatively charged N-terminal tail of dBigH1 alters the functional state of active chromatin compromising transcription.

| S-EPMC7192587 | biostudies-literature

The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-02-20 | GSE127227 | GEO

The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [ChIP1]

Project description:The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [ChIP1]

| PRJNA524366 | ENA

The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [ChIP2]

Project description:The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [ChIP2]

| PRJNA524367 | ENA

The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [Affy]

Project description:The embryonic linker histone dBigH1 compromises the functional epigenetic state of active chromatin [Affy]

| PRJNA524368 | ENA

Genomic distribution of dBigH1 in whole testes [ChIP-Seq]

Project description:This analysis largely reflected the genomic distribution of dBigH1 in spermatocytes since the vast majority of dBigH1-expressing cells in testes correspond to spermatocytes. In these studies, we observed that dBigH1 was not uniformely distributed across the genome.

2017-12-13 | GSE96959 | GEO

Genomic distribution of dBigH1 in early embryos [ChIP-Seq]

Project description:This analysis largely reflected the genomic distribution of dBigH1 in 0-2hpf early embryos. In these studies, we observed that dBigH1 was uniformely distributed across the genome.

2017-12-13 | GSE103291 | GEO

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