Transcriptomics

Dataset Information

0

Breast cancer cell line panel stimulated with estradiol


ABSTRACT: A panel of twelve breast cancer cell lines (ATCC) were exposed to 0, 1 or 10 nM 17β-estradiol (E2) for 16 hours. Cells were maintained in DMEM/F12 1:1 (DF) supplemented with 10% or 20% fetal calf serum (FCS) or 20% FCS and 0.01 mg/ml insulin. Two days before experiments, cells were seeded in 60 mm plates in DF with charcoal stripped phenol red-free serum supplemented with non-essential amino-acids and pen/strep, at a density such that 75%-90% confluence was obtained by the time of harvesting. Medium was refreshed 24 hours before exposure to 1 nM E2, 10 nM E2 or DMSO (control) for 16h. Subsequently cells were harvested and RNA was extracted using the Nucleospin RNA isolation kit (Macherey-Nagel).

ORGANISM(S): Homo sapiens

PROVIDER: GSE127760 | GEO | 2019/06/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-11-30 | MSV000083175 | MassIVE
2022-06-20 | PXD030843 | Pride
2022-06-20 | PXD031041 | Pride
2004-12-16 | GSE2071 | GEO
2004-12-08 | E-MEXP-215 | biostudies-arrayexpress
2016-06-01 | PXD004085 | Pride
2004-06-01 | GSE1131 | GEO
2020-07-01 | GSE148124 | GEO
2020-07-13 | PXD004648 | Pride
2016-01-29 | E-GEOD-77338 | biostudies-arrayexpress