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Muscle transcriptional profiles of chickens from slow and fast growing lines with different degree of WS and WB myopathies

ABSTRACT: Broiler industry is facing an increasing prevalence of breast myopathies such as white striping (WS) and wooden breast (WB), whose precise etiology remains poorly understood. In order to progress in the understanding of the structural changes and the molecular pathways involved in these myopathies, and identify biomarkers, a transcriptomic analysis using an 8×60K Agilent chicken microarray was performed. The study used pectoralis major muscles from three groups: slow-growing animals (n=8), fast-growing animals visually free from defects (n=8) or severely affected by both WS and WB (n=8). In addition, a weighted correlation network analysis was performed to investigate the relationship between modules of co-expressed genes and histological traits.

ORGANISM(S): Gallus gallus

PROVIDER: GSE127806 | GEO | 2019/10/18

REPOSITORIES: GEO

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Project description:Introduction: Skeletal muscle satellite cells (MuSCs, or stem cells) play a crucial role in muscle development, maintenance, and regeneration, serving both hypertrophic support and regenerative myogenesis. Syndecans (SDCs) act as communication bridges within the muscle microenvironment, regulating interactions with extracellular matrix components, and contributing significantly to tissue repair and inflammation. Specifically, syndecan-4 (SDC4) is involved in muscle regeneration at multiple stages. Methods: This study delves into the emerging challenge of wooden breast (WB) myopathy and its connection to SDC4. Our hypothesis proposes that disruptions in MuSC dynamics through SDC4 contribute to the increased incidence of breast myopathies observed in growing broilers. To test our hypothesis, non-affected and affected broilers were systematically selected, and the characteristics of WB myopathy were studied both in vitro and in vivo. SDC4 overexpression in MuSCs and blocking peptides corresponding to the SDC4 ectodomain were used for investigation of the role of SDC4 in muscle development and its shedding levels. Results and Discussion: Examination in vivo of affected muscles revealed smaller fibers and changes in metabolic pathways. In vitro studies unveiled disrupted proliferation of MuSCs in WB myopathy, accompanied by downregulation of several muscle markers. Investigation of a potentially role of SDC4 in the pathogenesis of WB myopathy revealed a decreased tendency in SDC4 gene expression and increased shedding of its ectodomain. Moreover, we showed that SDC4 overexpression is linked to decreased proliferation in MuSCs and affected myogenesis. We detected the impairment proliferation of WB-affected MuSCs, revealing critical insights into the dysfunctional state of these cells in myopathy. Additionally, by treating MuSCs with blocking peptides derived from the SDC4 ectodomain, we identified altered proliferation. Taken together, this work contributes valuable knowledge on the molecular mechanisms underlying WB myopathy and role of SDC4 in this chicken myopathy.

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Muscle transcriptional profiles of chickens from slow and fast growing lines with different degree of WS and WB myopathies

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