Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing to compare gene expession in control and PF228-treated hepatic stellate cells

ABSTRACT: Primary human hepatic stellate cells (HSCs) isolated from healthy donors were purchased from ScienCell Research Laboratories. They were preincubated with or without PF228 and stimulated with TGFbeta1 (5 ng/ml) for 24 hours. Cells were collected for RNA isolation and RNA sequencing. The goal of this study was to identify genes transcriptionally regulated by TGFbeta1 and FAK.

ORGANISM(S): Homo sapiens

PROVIDER: GSE127964 | GEO | 2019/04/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA sequencing to compare gene expression in control and Glut1 knockdown hepatic stellate cells

Project description:Primary human hepatic stellate cells (HSCs) isolated from healthy donors were purchased from Sciencell. HSC cells were transfected with viruses encoding control or Glut1 shRNA and stimulated with TGFbeta1 (5 ng/ml) for 24 hours. Cells were collected for RNA isolation and RNA sequencing. The goal of this study is to identify genes transcriptionally regulated by Glut1.

2024-05-31 | GSE223602 | GEO

RNA sequencing to compare gene expression in control and PD-L1 knockdown hepatic stellate cells

Project description:Primary human hepatic stellate cells (HSCs) isolated from healthy donors were purchased from Sciencell. They were transduced with viruses encoding control or PD-L1 shRNA and stimulated with TGFbeta1 (5 ng/ml) for 24 hours. Cells were collected for RNA isolation and RNA sequencing. The goal of this study is to identify genes transcriptionally regulated by PD-L1.

2021-12-08 | GSE167173 | GEO

RNA sequencing to compare gene expession in hepatic stellate cells on a soft (400 Pa) and stiff (25000 Pa) polyacrylamide hydrogels

Project description:Primary human hepatic stellate cells (HSCs) isolated from healthy patients were purchased from Sciencell. They were seeded on either 400 Pa or 25000 Pa polyacrylamide hydrogels for 2 days and collected for RNA isolation. The goal of this study is to determine genes transcriptionally regulated by a stiff matrix. 2 RNA samples of HSCs on 400 Pa and 3 RNA samples of HSCs on 25600 Pa were sent to the University of Minnesota Genomics Center for RNA sequencing.

2017-07-18 | GSE101343 | GEO

Gene expression analysis of hepatic stellate cells using Illumina HumanHT-12_V4 beadchip

Project description:Human primary hepatic stellate cells were purchased from ScienCell Research Laboratories. To identify TGFβ transcription targets and those are also modulated by p300 acetyltransferase, cells were treated with C646 to inhibit p300 acetyltransferase or transduced with p300 shRNA to knockdown p300. Thus, cell were divided into 4 groups: cells treated with DMSO, cells treated with C646 (15 uM), cells transduced with control shRNA, and cells transduced with p300 shRNA. After cells were serum-starved, they were stimulated with or without TGF-beta for 24 hours. Total RNA was then extracted and sent to University of Minnesota Genomic Center for microarray analysis.

2018-07-03 | GSE116509 | GEO

Effects of dexamethasone for primary trabecular meshwork cell gene expression

Project description:To clarify the effects of dexamethasone treatment for primary trabecular meshwork cell gene expression, which may relates to the pathophysiology of glucocorticoid-induced glaucoma Three lots (lot #2584, 3423 and 4973) of primary culture human trabecular meshwork (TM) cells were purchased from ScienCell Research Laboratories (Carlsbad, CA). The TM cells were treated with and without 100nM dexamethasone (DEX) for 14 days. Genomewide gene expression analysis was carried out using Agilent 8X60K array.

2015-01-24 | E-GEOD-65240 | biostudies-arrayexpress

RNA sequencing to compare gene expession in control and PF228-treated hepatic stellate cells

Project description:RNA sequencing to compare gene expession in control and PF228-treated hepatic stellate cells

| PRJNA525881 | ENA

The effect of postoperative stellate ganglion block on myocardial injury in patients undergoing laparoscopic radical resection of colorectal cancer

Project description:Interventions: Block Group:Stellate ganglion block;Routine Group:Saline injection around the stellate ganglion Primary outcome(s): Troponin I Study Design: Parallel

| 35777 | ecrin-mdr-crc

Deactivation of Hepatic Stellate Cells during Liver Fibrosis Resolution in Mice

Project description:Gene expression of mouse hepatic stellate cells was characterized under the following conditions: Quiescent (isolated from normal mouse liver) and reverted (isolated from mouse liver treated with 4 injections of carbontetrachloride followed by 45 day rest period) Affymetrix Mouse 1.0ST gene expression measurements were used to characterize the transcriptomic basis in quiescent hepatic stellate cells, isolated from normal liver, and reverted hepatic stellate cells, isolated from liver treated with 4 injections of CCl4 followed by a 45 day rest period. Gene expression of mouse hepatic stellate cells was characterized under the following conditions: A. Quiescent control hepatic stellate cells (n=4). B. Reverted hepatic stellate cells (n=4).

2014-05-05 | E-GEOD-38648 | biostudies-arrayexpress

Expression data from pancreatic stellate cells

Project description:tumor-stroma crosstalk drives pancreatic carcinogenesis we used time-resolved genome-wide transcriptional profiling to analyse changes caused by co-exposure of pancreatic tumor and stellate cells Primary pancreatic Stellate cells (PSC) were treated with a cumulative supernatant of pancreatic tumor cell lines (n=8) and harvested at 1-7, and 24 hours post exposure for RNA extraction and hybridization on Affymetrix microarrays. The 8 tumor cell lines are pancreatic ductal adenocarcinoma lines: AsPC1, BxPC3, Capan1, Colo357, MiaPaca2, Panc1, Su8686, and T3M4

2013-08-09 | E-GEOD-49583 | biostudies-arrayexpress

Early Mouse Hepatic Stellate Cell Activation

Project description:Early during culture of primary mouse HSCs gene expression changes. These expression alterations can be affected by treating cells with histone deacetylase inhibitor, valproic acid Primary mouse Hepatic stellate cells were cultured for short periods of time (4-16-64h) in presence or absence of valproic acid. Gene expression analysis (mouse Gene 1.0 ST arrays according to manufacturerM-bM-^@M-^Ys manual 701880Rev4 (Affymetrix, Santa Clara, CA)), in vitro stellate cell activation and inhibition of the activation by valproic acid treatment.

2014-01-01 | E-GEOD-51882 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data