Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CEA_SGF:E00009#switch_lympho_mega

ABSTRACT: we analyzed the gene expression profile of newly produced transgenic bone marrow subpopulations and compared it to the expression patterns obtained from cells isolated from wild type mice at different stages of the hematopoietic differentiation process. These populations were enriched in HSC, CMP, CD41+ c-kit+ and CD41+ c-kit- populations for the myelo-megakaryocytic lineage; and for the B lymphoid lineage, the populations were enriched in CLP, Pro-B and mature B lymphocytes. To produce comparable profiles total bone marrow RNA was used as a common reference. Keywords: repeat sample

ORGANISM(S): Mus musculus

PROVIDER: GSE1289 | GEO | 2004/12/01

SECONDARY ACCESSION(S): PRJNA90203

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

CEA_SGF:E00009#switch_lympho_mega

Project description:we analyzed the gene expression profile of newly produced transgenic bone marrow subpopulations and compared it to the expression patterns obtained from cells isolated from wild type mice at different stages of the hematopoietic differentiation process. These populations were enriched in HSC, CMP, CD41+ c-kit+ and CD41+ c-kit- populations for the myelo-megakaryocytic lineage; and for the B lymphoid lineage, the populations were enriched in CLP, Pro-B and mature B lymphocytes. To produce comparable profiles total bone marrow RNA was used as a common reference.

2010-06-05 | E-GEOD-1289 | biostudies-arrayexpress

CEA_SGF:E00008#transgenic_IIb-tk

Project description:To investigate genes that are involved in hematopoietic cells production in vivo, we used microarray technology combined with a transgenic mouse model IIb-tk previously developed in our laboratory (Tropel et al., 1997). In these mice, the promoter region of the murine CD41 (GPIIb) gene was used to target the expression of the thymidine kinase (tk) toxigene in cells specifically expressing the CD41 gene. The strength of the model resides in the fact that the depletion of the entire myelo-megakaryocytic lineage including stem cells/early progenitors by ganciclovir (GCV) administration could be inverted by discontinuing the treatment. A nearly full recovery of mature myelo-megakaryocytic cells is then observed within 3 days. To determine at the molecular level how BM cells could be regenerated in vivo following the eradication of all myeloid progenitors by the GCV treatment, microarray technology was applied. The strategy involved monitoring early transcriptional changes for the first 3-day of BM regeneration. Keywords: time-course

2004-04-09 | GSE1290 | GEO

CEA_SGF:E00008#transgenic_IIb-tk

2010-06-09 | E-GEOD-1290 | biostudies-arrayexpress

Fate mapping reveals two pathways of native thrombopoiesis (bulk RNA sequencing)

Project description:Bulk transcriptomes of Megakaryocyte progenitor cells isolated from bone marrow of WT mice; the following cell types were sorted: CD48high Megakaryocyte Progenitor (MkP): lineage- Sca-1- c-kit+ CD41+ CD150+ CD48hi; CD48-/low Megakaryocyte Progenitor (MkP): lineage- Sca-1- c-kit+ CD41+ CD150+ CD48lo

2022-04-28 | GSE183409 | GEO

Fate mapping reveals two pathways of native thrombopoiesis (scRNA sequencing)

Project description:Transcriptomes of 1141 single cells isolated from bone marrow of induced R26rtTA/rtTA/Col1A1H2B-GFP/H2B-GFP mice; the following cell types were sorted: Megakaryocyte Progenitor (MkP): lineage- Sca-1- c-kit+ CD41+ CD150+; Multipotent Progenitor (MPP): lineage- Sca-1+ c-kit+ CD48- CD150- ; Hematopoietic Stem Cell (HSC):lineage- Sca-1+ c-kit+ CD48- CD150+; E34 HSC: lineage- Sca-1+ c-kit+ CD48- CD150+ CD201hi CD34-/lo; Restricted hematopoietic progenitor 1 (HPC-1): lineage- Sca-1+ c-kit+ CD48+ CD150-; lineage- kit+ CD41- CD150- cells; lineage- kit+ CD150+ cells.

2022-04-28 | GSE159390 | GEO

Early innate lymphoid cell progenitor

Project description:We identified a new type of bone marrow progenitors termed early innate lymphoid cell progenitor (EILP) using TCF-1 GFP reporter mice. We compared the transcriptomes of early innate lymphoid cell progenitors (EILP) with other early progenitors, including HSC, LMPP, CMP, CLP, ETP and DN3.

2015-08-17 | GSE69789 | GEO

Gene expression profiles of human hematopoietic progenitors [BLUEPRINT hematopoietic progenitors]

Project description:An optimized whole genome bisulfite sequencing protocol (µWGBS, Farlik et al. 2015 Cell Reports) was used to establish DNA methylation profiles of FACS-purified stem and progenitor cells types of the human blood lineage. Most cell types were sorted from the peripheral blood of three donors each (43 donors total) with eight pools of ten cells, two pools of 50 cells, and one pool of 1000 cells. Additionally, two cell types (HSC, MPP) were sorted from bone marrow, fetal liver, and cord blood; single-cell methylome sequencing (scWGBS, Farlik et al. 2015 Cell Reports) was done for selected cell types (HSC, MPP, CMP, GMP, CLP, MLP0); and gene expression profiling using the Smart-seq2 protocol (Picelli et al. 2013 Nature Methods) was done on all stem/progenitor cell types (HSC, MPP, CMP, MEP, GMP, CLP, MLP0, MLP1, MLP2, MLP3).

2016-11-06 | GSE87195 | GEO

DNA methylation profiles of human hematopoietic progenitors [BLUEPRINT hematopoietic progenitors]

2016-11-06 | GSE87196 | GEO

Expression analysis of GATA1s murine megakaryocyte progenitors from bone marrow and fetal Liver

Project description:About 10% of Down syndrome (DS) infants are born with a myeloproliferative disorder (DS-TMD) that spontaneously resolves within the first few months of life. About 20-30% of these infants subsequently develop acute megakaryoblastic leukemia (DS-AMKL). In order to understand differences that may exist between fetal and bone marrow megakaryocyte progenitor cell populations we flow sorted megakaryocyte progenitor cells and performed microarray expression analysis. kewywords: Mouse megakaryocyte progenitors Expression data of flow cytometrically isolated murine megakaryocyte progenitor cells (lin-, Sca-1-, c-kit+, CD150+, CD41+) from GATA1s fetal liver and bone marrow

2013-03-29 | E-GEOD-45619 | biostudies-arrayexpress

Regulation of erythropoiesis in the spleen by Desert Hedgehog (Dhh)

Project description:Dhh negatively regulates multiple stages of erythrocyte differentiation. In Dhh-deficient bone marrow, the common myeloid progenitor (CMP) population was increased, but differentiation from CMP to granulocyte/macrophage progenitor was decreased, and the mature granulocyte population was decreased, compared with wild-type (WT). In contrast, differentiation from CMP to megakaryocyte/erythrocyte progenitor was increased, and the megakaryocyte/erythrocyte progenitor population was increased. In Dhh-deficient spleen and bone marrow, BFU-Es and erythroblast populations were increased compared with WT. During recovery of hematopoiesis after irradiation, and under conditions of stress-induced erythropoiesis, erythrocyte differentiation was accelerated in both spleen and bone marrow of Dhh-deficient mice compared with WT. To investigate possible mechanisms for its regulation of erythropoiesis we carried out RNAsequencing on Facs-sorted erythroblast population II (CD71+Ter119+) cells from Dhh-/-, Dhh+/- and WR mice.

2022-05-31 | GSE205034 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data