Gene expression analysis during differentiation of EPC to OEC
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE12891 | GEO | 2008/09/24
SECONDARY ACCESSION(S): PRJNA110885
REPOSITORIES: GEO
Similar Datasets
Project description:Gene expression profiles during the differentiation of EPCs into OECs were analyzed. Experiment Overall Design: RNA was isolated from Human cord blood derived- EPC and differentiated OEC from EPC. HG-U133A 2.0 microarray (54675 human genes; Affymetrix Santa Clara, CA, USA) was performed.
2008-10-26 | E-GEOD-12891 | biostudies-arrayexpress
Project description:Analysis of DNA methylation and gene expression changes during regulated endothelial progenitor cells (EPCs) to outgrowth endothelial cells (OECs). Results provide information of DNA methylation and gene expression pattern during cord-blood derived EPCs differentiation. Taken together, we discovered specific set of genes regulated by hyper- and hypo-methylation during differentiation.
2017-07-25 | GSE84774 | GEO
Project description:Analysis of DNA methylation and gene expression changes during regulated endothelial progenitor cells (EPCs) to outgrowth endothelial cells (OECs). Results provide information of DNA methylation and gene expression pattern during cord-blood derived EPCs differentiation. Taken together, we discovered specific set of genes regulated by hyper- and hypo-methylation during differentiation.
2017-07-25 | GSE84773 | GEO
Project description:Transcription profiling of human umbilical cord blood derived Outgrowth Endothelial Cells (OECs) at early and late passages. Outgrowth endothelial cells were isolated from the mononuclear fraction of umbilical cord blood and cultured on collagen coated flasks. Once OEC colonies emerged they were expanded in culture to study cell growth kinetics. Total RNA was extracted from OECs at an early passage and OECs at a late passage which were becoming senescent . The purpose of this experiment was to were compared early and late passage OECs to determine how senescence affects the gene expression profile of OECs.
2013-03-16 | E-MTAB-1388 | biostudies-arrayexpress
Project description:The largest germinal niche of the adult mammal brain locates at the ventricular zone (VZ), which is made up of adult neural stem cells (NSCs) and multiciliated ependymal cells (EPCs). Both NSCs and EPCs derive from radial glia (RG), whereas the transcriptomic dynamic changes of the cell fate continuum remain elusive. Here, we used TFEB ChIP-seq to investigate its role in during EPC differentiation.
2024-04-21 | E-MTAB-13855 | biostudies-arrayexpress
Project description:The largest germinal niche of the adult mammal brain locates at the ventricular zone (VZ), which is made up of adult neural stem cells (NSCs) and multiciliated ependymal cells (EPCs). Both NSCs and EPCs derive from radial glia (RG), whereas the transcriptomic dynamic changes of the cell fate continuum remain elusive. Here, we identified a key transcription factor TFEB that is important during EPC differentiation. We knockdown TFEB using siRNA and check gene expression changes upon TFEB depletion by RNA-seq.
2024-04-21 | E-MTAB-13856 | biostudies-arrayexpress
Project description:Analysis of regulators of endothelial progenitor cells (EPCs) differentiation at gene expression level. Results provide information of gene expression pattern and critical biological processing during EPCs differentiation. Taken together, we define the network of regulators of cord blood-derived EPCs during EPCs differentiation, which can be used to identify genes involved in vascular pathology. Furthermore, we select the critical regulators and then observe these selected regulator's functionality in vitro and in vivo. Total RNA obtained from isolated cord blood-derived EPCs at different time points after the beginning of the EPCs culture under endothelial differentiation condition.
2013-12-31 | E-GEOD-46328 | biostudies-arrayexpress
Project description:Analysis of regulators of endothelial progenitor cells (EPCs) differentiation at gene expression level. Results provide information of gene expression pattern and critical biological processing during EPCs differentiation. Taken together, we define the network of regulators of cord blood-derived EPCs during EPCs differentiation, which can be used to identify genes involved in vascular pathology. Furthermore, we select the critical regulators and then observe these selected regulator's functionality in vitro and in vivo.
2013-12-31 | GSE46328 | GEO
Project description:Background: In recent times a subset of bone marrow (BM) derived cells; endothelial progenitor cells (EPCs), have generated tremendous interest, as these cells are suggested to home to sites of neovascularization and neoendothelialization and differentiate into mature endothelial cells (ECs), a process referred to as postnatal vasculogenesis. EPCs are being considered as a potential regenerative tool for treating various pathophysiological disease states including cardiovascular disorder and as a possible target to restrict vessel growth in tumour pathology. However, conflicting results have been reported in the field due to lack of EPC specific biomarkers, and the identification, characterization, and the precise role of EPCs in vascular biology still remains a subject of great debate. Therefore, the objective of this study was to use a bioinformatics approach to identify putative novel EPC specific biomarkers. Methods: This study reports a detailed gene expression profile of human umbilical cord blood (UCB) derived non-adherent CD133 + cells at different time points during in vitro differentiation (day 4 and day 7) which were compared with differentiated donor matched human umbilical vein endothelial cells (HUVEC). Results: EPC gene expression was profiled at both days 4 and 7, using affymetrix human 1.0ST exon arrays. Affymetrix gene expression profiling revealed significant expression changes in genes associated with stem/progenitor cell properties such as adhesion, signalling, molecular transport, cell structure organisation and growth. Conclusions: This is the first study utilizing gene expression profiling to examine non adherent CD133+ progenitor cells. Alterations in the gene expression reported in this study may be involved in the cellular processes characteristic of EPC development and differentiation. Gene expression profiling was performed on EPCs cultured for D4 and D7, on Affymetrix human 1.0ST exon array chips. Gene Spring (version GX11) was used to perform the gene expression analysis on three experimental groups; (a) D4 EPCs versus HUVEC; (b) D7 EPCs versus HUVEC and (c) D4 versus D7 EPCs.
2012-07-30 | E-GEOD-25979 | biostudies-arrayexpress
Project description:Genome -wide analysis of EPC subtypes obtained from both umbilical cord and peripheral blood. This experiment sought to compare the expression levels of eEPCs and OECs against monocytes and human dermal microvascular cells (HDMECs) in order to establish if these cells are linked to a monocytic or endothelial lineage. This is very important as the term EPC is currently used to describe cell populations which are quite dissimilar in terms of biological properties.
2010-03-01 | GSE20283 | GEO