Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TGF-β and RREB1 coordinate EMT programs in NMuMG cells

ABSTRACT: Epithelial-to-mesenchymal transitions (EMT) play prominent roles during development, regeneration and tumor progression. EMTs are triggered by TGF-β, RAS and other signals that cooperatively induce the expression of master EMT transcription factors such as SNAIL. Here, we elucidate how the TGF-β and RAS pathways jointly trigger EMTs and tie them to broader developmental programs. We identify RAS response element binding protein 1 (RREB1) as a critical partner of TGF-β-activated SMAD transcription factors in driving SNAIL expression and EMT program in mammary gland epithelial cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE128958 | GEO | 2020/01/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

TGF-β and RREB1 coordinate EMT programs in development and cancer

Project description:Epithelial-to-mesenchymal transitions (EMT) play prominent roles during development, regeneration and tumor progression. EMTs are triggered by TGF-β, RAS and other signals that cooperatively induce the expression of master EMT transcription factors such as SNAIL. Here, we elucidate how the TGF-β and RAS pathways jointly trigger EMTs and tie them to broader developmental programs. We identify RAS response element binding protein 1 (RREB1) as a critical partner of TGF-β-activated SMAD transcription factors in driving SNAIL expression in pancreatic pre-malignant epithelial cells, lung adenocarcinoma cells, and embryonic stem cells. Moreover, SMADs and RREB1 also drive EMT-associated fibrogenic programs in epithelial cells and mesendoderm differentiation in pluripotent embryonic cells. These findings illuminate the orchestration of EMT associated programs in gastrulation, fibrosis, and cancer.

2020-01-09 | GSE118765 | GEO

TGF-β and RAS jointly unmask primed enhancers to drive metastasis (Mnase-Seq)

Project description:Epithelial-to-mesenchymal transitions (EMTs) and extracellular matrix (ECM) remodeling are distinct yet important processes during carcinoma invasion and metastasis. TGF-β and RAS, signaling through SMAD and RAS-responsive element-binding protein 1 (RREB1), jointly trigger expression of EMT and fibrogenic factors as two discrete arms of a common transcriptional response in carcinoma cells. Here we demonstrate that both arms form a program for lung adenocarcinoma pulmonary metastasis and identify chromatin determinants tying the expression of the constituent genes to TGF-β and RAS dual inputs. RREB1 binds near H4K16ac histone marks and histone H2A.Z-loaded nucleosomes at enhancers in the fibrogenic genes IL11, PDGFB, and HAS2 and the EMT transcription factor SNAI1, priming these enhancers for activation by a SMAD4-INO80 nucleosome remodeling complex in response to TGF-β. These regulatory properties set the fibrogenic EMT program apart from RAS-independent TGF-β gene responses and illuminate the operation and vulnerabilities of a transcriptional program that promotes metastatic outgrowth.

2024-09-26 | GSE256018 | GEO

TGF-β and RAS jointly unmask primed enhancers to drive metastasis (DRIPc-seq)

2024-09-26 | GSE256017 | GEO

TGF-β and RAS jointly unmask primed enhancers to drive metastasis (RNA-Seq)

2024-09-26 | GSE256019 | GEO

TGF-β and RAS jointly unmask primed enhancers to drive metastasis (ChIP-Seq)

2024-09-26 | GSE256015 | GEO

TGF-β and RAS jointly unmask primed enhancers to drive metastasis (ATAC-Seq)

2024-09-26 | GSE256013 | GEO

TGF-β and RREB1 coordinate EMT programs in NMuMG cells

Project description:TGF-β and RREB1 coordinate EMT programs in NMuMG cells

| PRJNA529490 | ENA

NMuMG cell response to TGF-beta and TGF-beta +2APB treatments

Project description:Recent studies demonstrate that Ca2+ signaling has an important role in EMT. Use of Ca2+ blockers such as 2APB can inhibit cell migration induced by TGF-β. Interestingly, we see an unexpected increase in Snail expression upon Ca2+ blocker treatment of both MCF10A and NMuMG cells; this increase is not observed with 2APB treatment alone. Therefore, we believe that 2APB plays a synergistic role with TGF-β in Snail induction. We propose to investigate the gene networks that change following 2APB +TGF-β treatment.

2018-07-31 | GSE98596 | GEO

TGF-β and RREB1 coordinate EMT programs in development and cancer

Project description:TGF-β and RREB1 coordinate EMT programs in development and cancer

| PRJNA486767 | ENA

Haploinsufficiency of RREB1 causes a Noonan-like syndrome via epigenetic reprogramming of RAS-MAPK pathway genes.

Project description:RREB1 is an epigenetic regulator of the RAS-MAPK pathway genes.

2020-03-13 | GSE146902 | GEO