Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Glucose metabolism drives epigenetic landscape transitions that dictate muscle stem cell function

ABSTRACT: We report the application of chromatin immunoprecpitation (ChIP) and assay for transposase accessible chromatin (ATAC) followed by sequencing to assay chromatin response to metabolic perturbation.

ORGANISM(S): Mus musculus

PROVIDER: GSE129259 | GEO | 2019/06/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Glucose Metabolism Drives Histone Acetylation Landscape Transitions that Dictate Muscle Stem Cell Function.

Project description:The impact of glucose metabolism on muscle regeneration remains unresolved. We identify glucose metabolism as a crucial driver of histone acetylation and myogenic cell fate. We use single-cell mass cytometry (CyTOF) and flow cytometry to characterize the histone acetylation and metabolic states of quiescent, activated, and differentiating muscle stem cells (MuSCs). We find glucose is dispensable for mitochondrial respiration in proliferating MuSCs, so that glucose becomes available for maintaining high histone acetylation via acetyl-CoA. Conversely, quiescent and differentiating MuSCs increase glucose utilization for respiration and have consequently reduced acetylation. Pyruvate dehydrogenase (PDH) activity serves as a rheostat for histone acetylation and must be controlled for muscle regeneration. Increased PDH activity in proliferation increases histone acetylation and chromatin accessibility at genes that must be silenced for differentiation to proceed, and thus promotes self-renewal. These results highlight metabolism as a determinant of MuSC histone acetylation, fate, and function during muscle regeneration.

| S-EPMC6788807 | biostudies-literature

Bos taurus ATAC-seq across 88 tissue samples to profile open chromatin regions in the cattle genome

Project description:We performed the assay for transposase-accessible chromatin using sequencing (ATAC-seq) using 88 tissue samples to profile open chromatin regions in the cattle genome.

2023-08-24 | E-MTAB-11825 | biostudies-arrayexpress

ATAC-seq of liver of a Holstein Friesian infant male.

Project description:We performed the assay for transposase-accessible chromatin using sequencing (ATAC-seq) on a liver sample collected from one Holstein Friesian healthy male at one month of age to profile open chromatin regions genome-wide to identify putative regulatory elements related to phenotype of interest.

2021-07-09 | E-MTAB-9872 | biostudies-arrayexpress

ATAC-seq data in mouse and zebrafish limbs

Project description:Assay for Transposable Accessible Chromatin (ATAC) reveals a genome wide view of areas of open chromatin at very high resolution, which are often associated with regulatory activity. The ATAC-seq technology uses a Tn5 transposase loaded with nex-generation sequencing primers in order to simultaneously fragment areas of open chromatin and ligate adapters.

2015-01-06 | E-GEOD-61065 | biostudies-arrayexpress

Identification of accessible chromatin regions in response to cold stress in Vitis amurensis

Project description:We report the Assay for Transposase-Accessible Chromatin with sequencing (ATAC-seq) for high-throughput profiling of accessible chromatin regions after cold treatment in Vitis amurensis

2021-11-12 | GSE122487 | GEO

5597e873-4b97-494f-8ca6-3ba129a345d5 - samples

Project description:This dataset contains the open chromatin profiles of 8 patient H3-K27M mutant DMGs utilizing the single-cell/nucleus assay for transposase-accessible chromatin using sequencing (snATAC-seq)

| EGAD00001010170 | EGA

Assay for Transposase-Accessible Chromatin using sequencing of spleen memory CD4+ T cells

Project description:Assay for Transposase-Accessible Chromatin using sequencing of spleen memory CD4+ T cells

| PRJNA579595 | ENA

ATAC-seq for Pantr2 knockout cortical neurospheres

Project description:We used assay for transposase accessible chromatin with sequencing (ATAC-seq) to discover changes in chromatin accessibility in Pantr2 KO cortical neurospheres

2022-08-02 | GSE210135 | GEO

Cardiac differentiation of mESCs and analysis of chromatin accessibility time series

Project description:Assay for transposase-accessible chromatin-sequencing (ATAC-seq) was performed in a time-series of cardiac differentiation of mESCs

2024-09-02 | GSE183767 | GEO

The effect of INO80 on chromatin accessibility in the development of heart failure.

Project description:In order to detect the effect of INO80 on chromatin accessibility, we will utilize the Assay for Transposase Accessible Chromatin with highthroughput sequencing (ATAC-Seq).

2024-01-04 | GSE240178 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data