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Serum extracelluar vesicles miRNA in premature neonates with chronic lung disease (CLD) and non CLD

ABSTRACT: Serum were obtained from 4 groups, day0 and day28 neonates with CLD and non-CLD. Serum samples (250ul) from cord blood at birth and venous blood of neonates at 28 DAYS were transferred to sterile tubes containing 63 μl of ExoQuick precipitation solution (System Bioscience, Mountain View, CA, USA), and mixed. The mixtures were incubated for 30 min at 4℃, and centrifuged at 1500×g for 30 min. Then, the supernatants were removed, the pellets (extracellular vesicles) were diluted to 60 μl with phosphosaline buffer, and then thawed on dry ice for total RNA extraction with a miRvana isolation kit (Ambion). 3pM synthetic ath-miR159a was spiked into each sample to correct for technical differences. Using a human miRNA array, we identified 62 miRNAs that were universally expressed in CLD patients and non-CLD patients (Normalized Template).

ORGANISM(S): Homo sapiens

PROVIDER: GSE129400 | GEO | 2021/04/06

REPOSITORIES: GEO

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Serum extracelluar vesicles miRNA in premature neonates with chronic lung disease (CLD) and non CLD

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