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RNA proximity sequencing reveals properties of spatial transcriptome organization in the nucleus

ABSTRACT: Spatial transcriptomics aims to understand how the ensemble of RNA molecules in tissues and cells is organized in 3D space. Here we introduce Proximity RNA-seq, which identifies co-localization preferences for pairs or groups of chromatin-associated, nuclear-retained and nascent RNAs in cell nuclei. Proximity RNA-seq is based on massive-throughput RNA barcoding of sub-nuclear particles in water-in-oil emulsion droplets, followed by sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE129732 | GEO | 2019/05/03

REPOSITORIES: GEO

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