Other

Dataset Information

0

Exonuclease Requirements for Mammalian Ribosomal RNA Biogenesis and Surveillance


ABSTRACT: Ribosomal RNAs (rRNAs) biogenesis are multistep processes requiring the activity of several nuclear and cytoplasmic exonucleases. The exact processing steps for mammalian 5.8S rRNA remains obscure. Here, using loss-of-function approaches in mouse embryonic stem cells and deep sequencing of rRNA intermediates, we investigate at nucleotide resolution the requirements of exonucleases known to be involved in 5.8S maturation, and explore the role of the Perlman syndrome-associated 3’-5’ exonuclease Dis3l2 in rRNA processing. We expand the repertoire of Dis3l2 targets to three 5.8S, 5S, and 28S rRNAs. We uncover a novel cytoplasmic intermediate that we name ‘7SB’ rRNA that is generated through sequential processing by distinct exosome complexes. 7SB rRNA can be oligouridylated by TUT4/7 and subsequently processed by Dis3l2 and Eri1. Moreover, exosome depletion triggers Dis3l2-mediated decay (DMD) as a surveillance pathway for rRNAs. Our data identify previously unknown 5.8S rRNA processing steps and provide nucleotide level insight into the exonuclease requirements for mammalian rRNA processing.

ORGANISM(S): Mus musculus

PROVIDER: GSE129734 | GEO | 2019/04/13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-07-20 | PXD031592 | Pride
2021-09-09 | PXD023383 | Pride
2023-05-02 | GSE212930 | GEO
2017-12-18 | GSE96946 | GEO
2016-07-19 | E-GEOD-82336 | biostudies-arrayexpress
2011-03-01 | E-GEOD-23940 | biostudies-arrayexpress
2017-02-15 | GSE94889 | GEO
2016-12-07 | GSE90927 | GEO
2017-10-04 | GSE83821 | GEO
2018-12-29 | E-MTAB-7549 | biostudies-arrayexpress