Global analysis of enhancer targets: Bulk RNA-Seq validation

ABSTRACT: Single-cell perturbation assays such as Mosaic-seq enable highly multiplexed functional assessment of enhancers in their endogenous genomic context. By introducing a few computational and experimental improvements, we expanded the Mosaic-seq analysis to capture the secondary gene targets of enhancers. Our analysis of >500 putative enhancers in K562 cells demonstrates that many secondary hits are shared among enhancers targeting different transcriptional factors, which reveals an interwoven enhancer-driven gene regulatory network. Together, our data underscore the flexibility of manipulating gene transcription by modifying enhancer activity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE129825 | GEO | 2019/10/31

REPOSITORIES: GEO