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TCR and inflammatory signals tune human MAIT cells to exert specific tissue repair and effector functions

ABSTRACT: We sought to describe in detail human MAIT cell activation using a transcriptomic approach to define the basic transcriptome of MAIT cells in humans and to determine how this is modulated by activation. Fresh human peripheral blood cells were obtained from three donors, and were magnetically enriched on CD8+ cells, and flow-sorted for live CD161++TCR Va7.2+ MAIT cells. These were cultured in media for 20 hours (“unstimulated”) or in the presence of anti-CD3/CD28 TCR beads (T), cytokines (IL-12, 2ng/ml, IL-18, 50ng/ml, IL-15, 25ng/ml, and TL1A, 100ng/ml), or TCR beads and cytokines combined (TC).

ORGANISM(S): Homo sapiens

PROVIDER: GSE129906 | GEO | 2019/04/17

REPOSITORIES: GEO

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Single-cell analysis of human MAIT cell transcriptional, functional and clonal diversity [Exp 4]

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