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Effects of overexpression of EhMyb-dr on transcription in Entamoeba histolytica

ABSTRACT: A myb domain containing protein of the SHAQKY family, EhMyb-dr, was overexpressed in E. histolytica trophozoites, and effects on transcription were measured.

ORGANISM(S): Entamoeba histolytica

PROVIDER: GSE13023 | GEO | 2009/03/01

SECONDARY ACCESSION(S): PRJNA112565

REPOSITORIES: GEO

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Effects of overexpression of EhMyb-dr on transcription in Entamoeba histolytica

Project description:A myb domain containing protein of the SHAQKY family, EhMyb-dr, was overexpressed in E. histolytica trophozoites, and effects on transcription were measured. Two arrays of trophozoites expressing Myc tagged EhMyb-dr were compared to two arrays performed on wild type trophozoites. The effects of transfection and drug were controlled for by including data from trophozoites expressing irrelevant plasmids, and grown in the same level of G418. All parasites were of strain HM-1:IMSS

2009-03-01 | E-GEOD-13023 | biostudies-arrayexpress

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Effect of Rom1 silencing on gene expression in Entamoeba histolytica

Project description:Entamoeba histolytica trophozoites epigenetically silenced for ameobapore A (G3 parasites) were additionally silenced for the Rhomboid gene Rom1 (EHI_197460) using the mechanism outlined in Mirelman et al. Parasite. 2008 Sep;15(3):266-74 (PMID 18814693). Rom1 silencing was confirmed by RT-PCR. Gene expression in these parasites was compared to that of the parent G3 strain.

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