Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

WaGa single cell RNA sequencing

ABSTRACT: The Merkel cell carcinoma cell line (MCC) WaGa was sequenced using the 10x Genomics 3' Chromium v2.0 platform in order to analyse transcriptional heterogeneity on a single cell level

ORGANISM(S): Homo sapiens

PROVIDER: GSE130346 | GEO | 2019/04/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

WaGa single cell RNA sequencing of the MCC cell line WaGa under HDAC inhibitor treatment compared to untreated WaGa control cells

Project description:The Merkel cell carcinoma cell line (MCC) WaGa was sequenced without and under HDAC inhibitor treatment using the 10x Genomics 3' Chromium v2.0 platform. The treatment induced changes were analysed on a single cell level.

2019-12-12 | GSE141832 | GEO

Conversion of Sox2-Dependent Merkel Cell Carcinoma to a Differentiated Neuron-like Phenotype by T Antigen Inhibition

Project description:Keratinocyte-cocultured Merkel cell carcinoma cell line (MCC) CVG-1 cells transduced with contorl shRNA (shCtrl) and viral T antigen targeting shRNA (shpanT) were sequenced using the 10x Genomics platform in order to analyze transcriptional changes associated with MCC cancer cell differentiation into neuron-like cells on a single cell level.

2019-09-05 | GSE136867 | GEO

Single cell RNA-seq of human synovial joints from healthy donors

Project description:Human synovial Single cell RNA-seq was performed on three tissue samples from healthy donors. This experiment was done to explore the heterogeneity of cells in healthy human synovial joint and enabled the comparison of cellular states and composition to those of publicly available single cell RNA-seq datasets from psoriatic arthritis and rheumatoid arthritis patients. Human synovial cells were loaded immediately after tissue dissociation with up to 25,000 cells in a single well of a Chromium chip G (10x Genomics). 3’ gene expression libraries were generated using Chromium Next GEM Single Cell 3' Kit 3.1 with 3' Feature Barcode Kit and dual indexing (10x Genomics protocol CG000316 Rev C). Libraries were sequenced as paired end (PE) 150 bp by Illumina sequencing to 65-80% saturation. Reads were mapped to the GRCh38 human genome (GENCODE) using the 10x Genomics Cell Ranger pipeline (7.2.0).

2024-08-06 | E-MTAB-14339 | biostudies-arrayexpress

Single cell RNA-seq of WTC-CRISPRi human induced pluripotent stem cells

Project description:We performed 3' single-cell RNA-seq using the 10X Genomics Chromium (version 1 chemistry) system on ~19,000 undifferentiated human IPSCs to explore the cellular heterogeneity of a seemingly homogeneous cell population.

2018-08-17 | E-MTAB-6687 | biostudies-arrayexpress

scRNAseq reveals mechanisms of Merkel cell carcinoma acquired immunotherapy resistance [1]

Project description:Serial blood draws from a single patient and serial tumor biopsies from the same patient with metastatic Merkel cell polyomavirus Merkel cell carcinoma, who was treated with cellular immunotherapy targeting MCPyV (autologous endogenous T cell therapy) followed by checkpoint inhibitors (anti-PD1 and anti-CTLA4). The patient had a 22 month clinical response followed by late/acquired resistance. We performed scRNAseq using the 10x genomics 3' Chromium expression assay to determine potential mechanisms contributing to immunotherapy response and late resistance

2018-09-01 | GSE117988 | GEO

Single-cell RNA-Seq Analysis of Retinal Development Identifies NFI Factors as Regulating Mitotic Exit and Late-Born Cell Specification

Project description:Single cell RNA sequencing using either an adapted Smart-seq2 protocol on Chx10-GFP (+) retinal progenitor cells; 10x Genomics Chromium Single Cell system across 10 timepoints of mouse retinal development to examine retinal progenitor cell heterogeneity across retinal development and global changes in gene expression from early retinal neuroepithelial cells through specification and differentiation of retinal cell types; 10X Genomics Chromium Single Cell on P14 Nfia/b/x het control or Nfia/b/x tCKO (Chx10-Cre-GFP) retinas

2019-05-22 | GSE118614 | GEO

Single-cell RNA sequencing of human liver cells

Project description:To examine the heterogeneity and dynamic crosstalk of human liver cells, ~18,000 freshly isolated human liver cells from 6 donors were profiled using the 10x Genomics Chromium platform.

2021-09-29 | E-MTAB-10553 | biostudies-arrayexpress

scRNA-seq of mouse lymph node stromal cells

Project description:C57BL/6 mouse lymph node stromal cells were isolated for scRNA-seq, more than 2x104 cells were run on the 10X Chromium Controller (10X Genomics) to partition single cells with uniquely barcoded beads and processed for sequencing library preparation using the Chromium Single Cell 3’ Reagent Kit. cDNA libraries were sequenced on a NovaSeq 6000 sequencing system. 4 x 103 cells per sample were captured on the 10X Chromium chip. 5-10 x 104 reads/cell were obtained with characterization of 2-3 x 103 transcripts/cell.

2022-07-01 | GSE202068 | GEO

scRNA-seq of lymph node stromal cells from mouse treated with anti-CD40L

Project description:C57BL/6 mouse lymph node stromal cells treated with anti-CD40L were isolated for scRNA-seq, more than 2x104 cells were run on the 10X Chromium Controller (10X Genomics) to partition single cells with uniquely barcoded beads and processed for sequencing library preparation using the Chromium Single Cell 3’ Reagent Kit. cDNA libraries were sequenced on a NovaSeq 6000 sequencing system. 4 x 103 cells per sample were captured on the 10X Chromium chip. 5-10 x 104 reads/cell were obtained with characterization of 2-3 x 103 transcripts/cell.

2022-09-17 | GSE213400 | GEO

Gene expression profile at single cell level of three adult, non-diabetic, human islets

Project description:This data set contains 41,432 islet cells from three adult non-diabetic human islet donors (two female, one male) sequenced using 10X Genomics Chromium.

2024-06-15 | GSE269204 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data