Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of endogenous gene expression of HCT116 colorectal cancer cells and HCT116-derived DAPK1 knockout (ko) clones.

ABSTRACT: Data were obtained via Nanostring nCounter Gene Expression Assay (PanCancer Progression Panel, XT_PGX_HuV1_CancerProg_CSO XT-CSO-PROG1-12, Cat. no. 115000152, Nanostring Technologies, Hamburg, Germany) We aimed to reveal Death-associated protein kinase 1 (DAPK1)-dependent gene expression in the context of tumor progression covering genes involved in extracellular matrix, epithelial-mesenchymal transition, metastasis and angiogenesis comparing HCT116 DAPK1 wt cells and three different monoclonal DAPK1 ko clones named clone 7/6, clone 10/8 and clone 21/9. DAPK1 ko clones were generated by CRISPR/Cas9-mediated genomic editing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE130488 | GEO | 2019/05/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Endogenous expression profiles of HCT116 colorectal cancer cells and HCT116-derived ATF2 knockout (KO) clones [Version 02]

Project description:Gene expression profiles were obtained via NanoString nCounter Gene Expression Assay (PanCancer Progression Panel, XT_PGX_HuV1_CancerProg_CSO, cat. no. XT-CSO-PROG1-12, NanoString Technologies, Hamburg, Germany). We aimed to decipher the role of activating transcription factor 2 (ATF2) in colorectal cancer, so-far masked by high intratumoral heterogeneity (ITH). To overcome ITH, we generated monoclonal ATF2 knockout (KO) cells (termed F9 and E5) using CRISPR/Cas9 gene editing and analyzed alterations of their gene expression profiles in comparison to the parental HCT116 cell line (HCT116 WT).

2022-07-19 | GSE172488 | GEO

Expression profiles of HCT116 colorectal cancer cells and HCT116-derived CDKN1A (p21) knockout (ko) cells.

Project description:Gene expression profiles were obtained via Nanostring nCounter Expression Assay (PanCancer Progression Panel, Nanostring Technologies, Hamburg, Germany). We aimed to obtain a gene expression signature associated to the kockout of p21 (i.e. Cyclin Dependent Kinase Inhibitor 1A, CDKN1A) in colorectal carcinoma samples and its association with of epithelial-mesenchymal transition (EMT). We analysed and compared three independent cultures of HCT116 p21 wt cells and three HCT116 p21-/- ko cells.

2019-11-01 | GSE135923 | GEO

Expression profiles of SOX11-positive and SOX11-negative Mantle Cell Lymphoma (MCL) primary samples from lymph node and reactive lymph nodes (RLN).

Project description:Gene expression profiles were obtained via Nanostring nCounter Expression Assay (PanCancer Immune Profiling Panel, Nanostring Technologies, Hamburg, Germany). We aimed to obtain the differential immune gene expression comparing the lymph node microenvironment of SOX11-positive and SOX11-negative MCL primary samples and non-neoplastic nodal samples (RLN).

2021-12-01 | GSE141539 | GEO

Comparison of endogenous gene expression of HCT116 colorectal cancer cells and HCT116-derived DAPK1 knockout (ko) clones.

Project description:Comparison of endogenous gene expression of HCT116 colorectal cancer cells and HCT116-derived DAPK1 knockout (ko) clones.

| PRJNA540467 | ENA

Consensus molecular subtypes as biomarkers of FU/FA maintenance therapy with or without panitumumab in RAS wild-type metastatic colorectal cancer (PanaMa, AIO KRK 0212).

Project description:Customized Nanostring PanCancer Progression Panel performed on FFPE tumor tissue derived from treatment naive metastatic colorectal cancer patients included into the randomized phase 2 PanaMa trial.

2023-02-15 | GSE221953 | GEO

Tumour Infiltrating Lymphocytes and Immune-Related Genes as Predictors of Outcome in Pancreatic Adenocarcinoma

Project description:In this study, we analyzed a total of 12 patients affected by pancreatic tumour split into two different groups based on their outcomes (6 good vs 6 worse prognoses). After the IHC of different populations within cancer, we randomly selected 3 patients for each group and performed a gene expression analysis with Nanostring human PanCancer immune module.

2019-06-18 | E-MTAB-8079 | biostudies-arrayexpress

RNA-seq to compare gene expression profiles between Asf1a WT and Asf1a KO cells

Project description:KrasG12D/P53-/-(KP)-Cas9 single clone was transfected with ctrl vector, ctrl sgRNA-1, ctrl-sgRNA-2 or ctrl-sgRNA-3 and then selected by using 5ug/ml Blasticidin, to get the 4 ctrl lines; KP-Cas9 single clone was transfected with Asf1a sgRNA-1, sgRNA-2, sgRNA-3 and new sgRNA-1 and then selected by using 5ug/ml Blasticidin, and then picked up single clones with Asf1a KO. For each Asf1a sgRNA, we selected one clone for RNA seq, so totally we have 4 Asf1a KO clones for RNA seq.

2019-11-26 | GSE127205 | GEO

Immunological differences between primary and metastatic breast cancer

Project description:Little is known about how the immune microenvironment of breast cancer evolves during disease progression. The goal of this project was to examine immune gene mRNA expression in primary tumors (P) and metastatic lesions (M). The expression of 730 immune-related genes was measured using the Nanostring PanCancer Immune Gene Panel.

2018-08-20 | GSE102818 | GEO

Transcriptomic analysis of orthotopic pancreatic tumors treated by Axl inhibitor TP-0903 or combination with chemo/immunotherapy using the nCounter PanCancer Immune Profiling panel (Nanostring Technologies) [mouse]

Project description:RNA isolated from KPfC orthotopic tumors was analyzed using Mouse PanCancer Immune Profiling Panel (NanoString Technologies).

2021-11-01 | GSE185110 | GEO

Gene Expressions in OCCC using Nanostring analysis

Project description:Gene expression of OCC according to chemotherapeutic response was analyzed using Nanostring nCounter PanCancer Pathway Panel.

2022-09-05 | GSE212670 | GEO