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Gene profiles regulated and targeted by PRC2 in oligodendrocyte lineage cells

ABSTRACT: The PRC2 complex creates a repressive mark histone-H3-Lys27-trimethylation. Although PRC2 is involved in various biological processes, its role in glial development remains ambiguous. We find PRC2 complex is required for oligodendrocyte (OL) differentiation and myelination, but not for OL precursor formation. Furthermore, most PRC2-null OL lineage cells (cKO) acquire astrocyte features. RNA profiles from RNAseq assay reveal that many transcription factors involved in oligodendrocyte and astrocyte development are regulated by PRC2. Moreover, PRC2-null OL lineage cells aberrantly induce Notch-NFIA and Wnt pathway genes. The chromatin immunoprecipitation-sequencing (ChIPseq) assay further reveals that PRC2 is recruited to many of Notch-NFIA and Wnt pathway genes. The datasets demonstrate the molecular program underlying the specific action of PRC2 and suggest PRC2 as a gatekeeper that determines the timing of OL lineage progression and myelination.

ORGANISM(S): Mus musculus

PROVIDER: GSE130628 | GEO | 2020/09/15

REPOSITORIES: GEO

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