Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MRNA expression profiling in MDA-MB-231 (LM1) cells with a tet-incible MBD2 or p66α knock down, or treated with MBD2-targeting small molecule ABA or APC

ABSTRACT: The goal of this study is to compare mRNA expression profiles among wild type, MBD2 knock down, p66α knock down, and ABA- and APC-treated cells.. All cell lines were maintained in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. For the doxycycline inducible gene expression regulation, each cell lines were incubated in the presence of 1 μg/ml doxycycline for 2 days. For the drug treatment, MDA-MB-231 (LM1) cells were incubated in the presence of 10 μM ABA or APC for 2 days. Total cellular RNAs were extracted using Qiazol reagent. The RNA quality was examined by spectrophotometry, agarose gel electrophoresis (calculating the 18S and 28S rRNA ratio) and an Agilent Technologies 2100 Bioanalyzer (ensuring a RIN value greater than 7). Library was prepared with QuantSeq 3’ mRNA-Seq Library Prep Kit FWD. The constructed libraries were subjected to 75-bp single-end sequencing using an Illumina NextSeq 500 sequencer. Using an optimized data analysis workflow, we mapped to the human genome (build hg19) and identified 25,737 transcripts in these cell lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE130637 | GEO | 2019/05/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

mRNA expression profiling in MDA-MB-231 and HepG2 cells with 2 µM of ACP52C peptide treatment for 0, 12, 24, and 48 hours

Project description:The goal of this study is to compare mRNA expression profiles of the ACP52C-treated cells in time dependent manner. All cell lines were maintained in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. For the drug treatment, MDA-MB-231 and HepG2 cells were incubated in the presence of 2 μM ACP52C for 0, 12, 24, and 48 hours. Total cellular RNAs were extracted using Qiazol reagent. The RNA quality was examined by spectrophotometry, agarose gel electrophoresis (calculating the 18S and 28S rRNA ratio) and an Agilent Technologies 2100 Bioanalyzer (ensuring a RIN value greater than 7). The library was prepared using the RNA seq3’ mRNA-Seq Library Prep Kit FWD. The constructed libraries were subjected to 150-bp two-end sequencing using an Illumina NextSeq 500 sequencer at Insilicogen. All procedures were performed following the manufacturer’s instructions. Tophat 2 and cufflinks were used for reads mapping and expected read counts, respectively. DEGs were analyzed using an edgeR program and functional annotation of genes were achieved using a DAVID program.

2021-05-25 | GSE175393 | GEO

mRNA expression profiling in MDA-MB-231 (LM1) cells with a tet-incible MBD2 or p66α knock down, or treated with MBD2-targeting small molecule ABA or APC

Project description:mRNA expression profiling in MDA-MB-231 (LM1) cells with a tet-incible MBD2 or p66α knock down, or treated with MBD2-targeting small molecule ABA or APC

| PRJNA540857 | ENA

Transcriptomic and functional analysis of ANGPTL4 overexpression in pancreatic cancer nominates targets that reverse chemoresistance

Project description:3'RNAseq FWD with or without gemcitabine treatment in pancreatic cancer cell lines with altered ANGPTL4 expression

2023-01-19 | GSE207359 | GEO

Arabidopsis thaliana

Project description:apc transcriptome-Transcriptome on Arabidopsis APC hypomorphic lines

| PRJNA156939 | ENA

RNA sequencing of young versus aged endothelial cells

Project description:Freshly dissected Thymus, Kidney, and Spleen from young and aged mice were subjected to collagenase digestion to prepare single-cell suspension for the isolation of endothelial cells. Endothelial cells form the single-cell suspension was isolated using a magnetic bead-based separation method using CD144 and Endomucin antibody. RNA from 150,000 cells from each group was isolated using the RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer’s instructions. The library preparation was performed using QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina.

2021-01-23 | E-MTAB-8941 | biostudies-arrayexpress

Methylation map of 8 neuroblastoma cell lines: NGS after MBD2-capture

Project description:8 neuroblastoma (NB) cell lines (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1) their methylome is determined by sequencing after MBD2-capture using MethylCollector (ActiveMotif) 8 NB cell lines were included (CLB-GA, IMR-32, SH-SY5Y, N206, CHP-902R, LAN-2, SK-N-AS, SJNB-1) in this study. After shearing (fragments of about 200 bp), DNA was captured using MBD2-capture (MethylCollector - ActiveMotif) followed by library preparation and multiplexing. Captured sequence tags were sequenced paired-end (2 x 45 bp) on Illumina GAIIx.

2012-09-13 | E-GEOD-31353 | biostudies-arrayexpress

ZHBTc4 ES cells stably expressing EGFP vs Nr5a2-EGFP or Oct4-EGFP vs Nr5a2-EGFP

Project description:Gene expression profiles of ZHBTc4 ES cells expressing EGFP, Oct4-EGFP, Nr5a2-EGFP under CAG promoter. Monoclonal cell lines selected by Puromycin were used for analysis. Each of the cell lines was cultured in doxycycline containing media for endogenous Oct4 knock-down. Pupose of this experiment is to investigate the possibility that forced expression of Nr5a2 can replace Oct4 function in the self-renewal of ES cells.

2013-11-05 | GSE52034 | GEO

Tissue specfic proteome quantitation and Temporal proteome profile of Arabidopsis upon ABA exposure using DIA-MS

Project description:Use of our recent spectral library, we quantify proteomes of 4 tissue type in arabidopsis, and also the proteomes in response to ABA-treatment. We revealed the temproal profiling of ABA-responding genes.

2019-09-02 | PXD014032 | Pride

Allele-specific binding analysis of rs242561 by NRF2 ChIP tagmentation sequencing

Project description:We identified a SNP rs242561, located within a regulatory region of the MAPT gene (encoding microtubule-associated protein Tau). It was consistently occupied by NRF2/sMAF in multiple ChIP-seq experiments, and its strong-binding allele increased transactivation, and accorded higher mRNA levels in cell lines and human brain. To confirm the allele-specific binding, we conducted ChIP tagmentaion sequencing experiment in human lymphoblastoid cell line GM12763 which is heterozygous for rs242561. NRF2 ChIP DNA was isolated from GM12763 cells treated with Sulforaphane (SFN) in triplicates. The region containing rs242561 was amplified using primers, Fwd 5’-AGCCTTCCCTGTCCTTGATT-3’, Rev 5’-GGACCGAGCTTCCAGTCTAA-3’, and tagmentated using Tn5-based transposition for library construction. Libraries were sequenced on Illumina MiSeq.

2016-04-14 | GSE79569 | GEO

Allele-specific binding analysis of rs242561 by NRF2 ChIP tagmentation sequencing

Project description:We identified a SNP rs242561, located within a regulatory region of the MAPT gene (encoding microtubule-associated protein Tau). It was consistently occupied by NRF2/sMAF in multiple ChIP-seq experiments, and its strong-binding allele increased transactivation, and accorded higher mRNA levels in cell lines and human brain. To confirm the allele-specific binding, we conducted ChIP tagmentaion sequencing experiment in human lymphoblastoid cell line GM12763 which is heterozygous for rs242561. NRF2 ChIP DNA was isolated from GM12763 cells treated with Sulforaphane (SFN) in triplicates. The region containing rs242561 was amplified using primers, Fwd 5â??-AGCCTTCCCTGTCCTTGATT-3â??, Rev 5â??-GGACCGAGCTTCCAGTCTAA-3â??, and tagmentated using Tn5-based transposition for library construction. Libraries were sequenced on Illumina MiSeq. NRF2 ChIP tagmentation sequencing of a 241bp intron region in MAPT gene using GM12763 treated with sulforaphane

2016-04-14 | E-GEOD-79569 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data