Single-cell transcriptional and proteomic profiling of tumor-infiltrating lymphocytes
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ABSTRACT: To characterize the transcriptional environment of neoepitope-specific TILs, we performed multiplexed quantitative PCR analysis on tetramer-positive and -negative CD8+ T cell populations populations; by using an index sorting approach, we were able to simultaneously assay transcription and cell-surface phenotype at single-cell resolution. The 88 genes we selected for analysis accounted for: transcription factors shown to influence CD8+ T cell differentiation, chemokines and chemokine receptors, cytokines, activating and inhibitory co-receptors, and molecules associated with degranulation and cell survival. We observed a spectrum of dynamic transcriptional changes that take place during antigen-specific CD8+ T cell responses, specifically identifying three clusters with distinct transcriptional signatures across the sorted TIL populations, and these clusters differed in their functional status and in the expression of transcription factors, transcriptional regulators, signaling inhibitors, cytokines, cytotoxic granules, and trafficking molecules.
ORGANISM(S): Homo sapiens
PROVIDER: GSE130670 | GEO | 2019/05/04
REPOSITORIES: GEO
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