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High-Throughput Single-Molecule R-loop Footprinting Reveals Principles of R-loop Formation


ABSTRACT: We have successfully characterized R-loop structures using non-denaturing bisulfite treatment coupled with single-molecule sequencing to reveal that R-loops form defined overlapping molecular clusters around promoter, gene body, and terminal regions of genes. Our results uncovers that R-loop formation is both driven by favorable DNA sequence as well as topological state.

ORGANISM(S): Escherichia coli Homo sapiens

PROVIDER: GSE130726 | GEO | 2019/09/20

REPOSITORIES: GEO

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