Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [K562]

ABSTRACT: Heat shock (HS) causes dramatic and rapid changes in transcription of thousands of genes in metazoans. We have used insitu Hi-C in Drosophila S2 and human K562 cells to determine the effects of HS upon chromatin conformation. Our study shows that topologically associated domains (TADs), compartments and distal regulatory interactions remain unchanged upon HS.

ORGANISM(S): Homo sapiens

PROVIDER: GSE130758 | GEO | 2019/06/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [S2]

Project description:Heat shock (HS) causes dramatic and rapid changes in transcription of thousands of genes in metazoans. We have used insitu Hi-C in Drosophila S2 and human K562 cells to determine the effects of HS upon chromatin conformation. Our study shows that topologically associated domains (TADs), compartments and distal regulatory interactions remain unchanged upon HS.

2019-06-07 | GSE130776 | GEO

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [S2]

Project description:Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [S2]

| PRJNA541321 | ENA

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock.

Project description:Heat shock (HS) initiates rapid, extensive, and evolutionarily conserved changes in transcription that are accompanied by chromatin decondensation and nucleosome loss at HS loci. Here we have employed in situ Hi-C to determine how heat stress affects long-range chromatin conformation in human and Drosophila cells. We found that compartments and topologically associating domains (TADs) remain unchanged by an acute HS. Knockdown of Heat Shock Factor 1 (HSF1), the master transcriptional regulator of the HS response, identified HSF1-dependent genes and revealed that up-regulation is often mediated by distal HSF1 bound enhancers. HSF1-dependent genes were usually found in the same TAD as the nearest HSF1 binding site. Although most interactions between HSF1 binding sites and target promoters were established in the nonheat shock (NHS) condition, a subset increased contact frequency following HS. Integrating information about HSF1 binding strength, RNA polymerase abundance at the HSF1 bound sites (putative enhancers), and contact frequency with a target promoter accurately predicted which up-regulated genes were direct targets of HSF1 during HS. Our results suggest that the chromatin conformation necessary for a robust HS response is preestablished in NHS cells of diverse metazoan species.

| S-EPMC6765289 | biostudies-literature

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-06-07 | GSE130778 | GEO

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock

Project description:Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock

| PRJNA541319 | ENA

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [K562]

Project description:Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [K562]

| PRJNA541309 | ENA

The host transcriptome remains unaltered during the establishment of coral-algal symbioses

2009-04-01 | GSE14923 | GEO

Stable Chromosome Condensation Revealed by Chromosome Conformation Capture

Project description:Chemical cross-linking and high-throughput sequencing have revealed regions of intra-chromosomal interaction, referred to as topologically associating domains (TADs), interspersed with regions of little or no such interaction, in interphase nuclei. We find that TADs and the regions between them correspond with the bands and interbands of polytene chromosomes of Drosophila. We further establish the conservation of TADs between polytene and diploid cells of Drosophila. From direct measurements on light micrographs of polytene chromosomes, we then deduce the states of chromatin folding in the diploid cell nucleus. Two states of chromatin folding, fully extended fibers containing regulatory regions and promoters, and fibers condensed up to ten-fold containing coding regions of active genes, constitute the euchromatin of the nuclear interior. Chromatin fibers condensed up to 30-fold, containing coding regions of inactive genes, represent the heterochromatin of the nuclear periphery. A convergence of molecular analysis with direct observation thus reveals the architecture of interphase chromosomes. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-11-05 | E-GEOD-72512 | biostudies-arrayexpress

Proximity labeling proteomics of ATG8

Project description:TurboID-mediated proximity labeling proteomics of ATG8 in Arabidopsis upon heat stress. The TurboID-YFP-ATG8a (TI-Y8a) and TurboID-YFP (TI-YFP) transgenic seedlings were harvested after 3 hrs of recovery from HS treatment for proximity proteomic analysis. The biotinylated proteins purified from HS-treated TI-Y8a plants were compared with proteins from HS-treated TI-YFP plants (TI-Y8a HS vs. TI-YFP HS) and those from HS-untreated TI-Y8a plants (TI-Y8a HS vs. TI-Y8a CK), respectively.

2023-01-23 | PXD039608 |

The host transcriptome remains unaltered during the establishment of coral-algal symbioses

Project description:Coral reefs are based on the symbiotic relationship between corals and photosynthetic dinoflagellates of the genus Symbiodinium. We followed gene expression of coral larvae of Acropora palmata and Montastraea faveolata after exposure to Symbiodinium strains that differed in their ability to establish symbioses. We show that the coral host transcriptome remains almost unchanged during infection by competent symbionts, but is massively altered by symbionts that fail to establish symbioses. Our data suggest that successful coral-algal symbioses depend mainly on the symbionts' ability to enter the host in a stealth manner rather than a more active response from the coral host. Acropora palmata Samples: Three biological replicates of pooled larvae from each species and condition (i.e. untreated control, inoculated with competent Symbiodinium strain, inoculated with incompetent Symbiodinium strain) for both time points were hybridized against a pooled reference. Pooled references were constructed by combining equal amounts of aRNA from all control samples from A. palmata. References were labeled with Cy3, samples with Cy5. Montastraea faveolata Samples: Three biological replicates of pooled larvae from each species and condition (i.e. untreated control, inoculated with competent Symbiodinium strain, inoculated with incompetent Symbiodinium strain) for both time points were hybridized against a pooled reference. Pooled references were constructed by combining equal amounts of aRNA from all control samples from M. faveolata. References were labeled with Cy3, samples with Cy5. Symbiodinium sp. CassKB8: competent strain Symbiodinium sp. EL1: incompetent strain Symbiodinium sp. Mf1.05b: competent strain

2010-06-20 | E-GEOD-14923 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data