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MRNA sequencing time course of C. elegans samples at time point 0 hours until time point 15 hours at 25 degree.

ABSTRACT: We find gene expression oscillations which arise after a time lag of around 5 hours.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE130782 | GEO | 2020/07/27

REPOSITORIES: GEO

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mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 24 hours at 25 degree [blmp-1(tm548) mutant]

Project description:We find gene expression oscillations which arise after a time lag of around 5 hours. Molecular oscillators can organize gene expression during development both temporally and spatially. In C. elegans, oscillations occur for ~25% of the transcriptome to control molting and potentially other developmental processes. The underlying mechanisms and organization of the oscillator remain unknown. We investigated the transcription factor BLMP-1, orthologous to mammalian PRDM1, as a rhythmically accumulating transcription factor that is required for robust rhythmic development, molting, and animal viability. We have sequenced synchronously growing populations of wild-type and blmp-1(tm548) mutant animals in a time course from 1 – 24 hours after plating with an hourly resolution.

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mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 24 hours at 25 degrees [N2 control]

Project description:We find gene expression oscillations which arise after a time lag of around 5 hours.

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mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 16 hours at room temperature

Project description:We find gene expression oscillations which arise after a time lag of around 7 hours.

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mRNA sequencing time course of C. elegans samples at time point 0 hours until time point 15 hours at 25 degree.

Project description:mRNA sequencing time course of C. elegans samples at time point 0 hours until time point 15 hours at 25 degree.

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mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 24 hours at 25 degree [blmp-1(tm548) mutant]

Project description:mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 24 hours at 25 degree [blmp-1(tm548) mutant]

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mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 24 hours at 25 degrees [N2 control]

Project description:mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 24 hours at 25 degrees [N2 control]

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mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 16 hours at room temperature

Project description:mRNA sequencing time course of C. elegans samples at time point 1 hours until time point 16 hours at room temperature

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mRNA-seq time course of C. elegans sampled from 5h until 48h at 25 degrees

Project description:We find 3739 'high-confidence oscillating' genes. These genes peak 4 times during larval development, but cease in adulthood

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Degree of Collaterals and Not Time Is the Determining Factor of Core Infarct Volume within 6 Hours of Stroke Onset.

Project description:Background and purposeGrowth of the core infarct during the first hours of ischemia onset is not well-understood. We hypothesized that factors other than time from onset of ischemia contribute to core infarct volume as measured by MR imaging.Materials and methodsProspectively collected clinical and imaging data of consecutive patients with stroke presenting between March 2008 and April 2013 with anterior circulation large-vessel occlusion and MR imaging performed within 6 hours from the time of onset were reviewed. The association of time from onset, clinical, and radiographic features with DWI volume was assessed by using χ(2) and Mann-Whitney U tests.ResultsOf 91 patients, 21 (23%) underwent MR imaging within 0-3 hours from onset, and 70 (76%), within 3-6 hours. Median MR imaging infarct volume was similar in both timeframes, (24.7 versus 29.4 mL, P = .906), and there was no difference in the proportion of patients with large infarct volumes (≥70 mL, 23.8% versus 22.8%, P = .928). Using receiver operating characteristic analysis, we detected no association between the time from onset and MR imaging infarct volume (area under the curve = 0.509). In multivariate analysis, CTA collaterals (>50% of the territory) (adjusted OR, 0.192; 95% CI, 0.04-0.9; P = .046), CTA ASPECTS (adjusted OR, 0.464; 95% CI, 0.3-0.8; P = .003), and a history of hyperlipidemia (adjusted OR, 11.0; 95% CI, 1.4-88.0; P = .023) (but not time from stroke onset to imaging) were independent predictors of MR imaging infarct volume.ConclusionsCollateral status but not time from stroke onset to imaging was a predictor of the size of core infarct in patients with anterior circulation large-vessel occlusion presenting within 6 hours from onset.

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Mnemiopsis leidyi high resolution developmental transcriptomic time-course 0-20 hours

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