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Ultra-High Throughput Single Cell Analysis of Proteins and RNAs by Split-pool Synthesis

ABSTRACT: We report a method enabling simultaneous, ultra-high throughput single-cell-barcoding, of millions of cells for targeted single cell analysis of proteins and RNAs. This method termed Quantum Barcoding (QBC) circumvents the need to isolate single cells by building cell-specific oligo barcodes dynamically within each cell. With minimal instrumentation (four 96-well plates and a multichannel pipette) cell-specific codes are added to each tagged molecule within cells. This is accomplished through sequential rounds of the well-established process of classic “split-pool synthesis”. We demonstrate the utility of this currently research use only technology in multiple model systems.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE130784 | GEO | 2019/05/07

REPOSITORIES: GEO

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